In vitro drug-drug interaction study between Ranitidine Hydrochloride and Bisoprolol fumarate

Abstract

The current study's objective is to assess the pharmacological interactions between Bisoprolol fumarate and Ranitidine hydrochloride using in vitro model. Using the Job's approach and Ardon's method, a spectral observation research, and the thin layer chromatography method, interactions between bisoprolol fumarate and ranitidine hydrochloride were assessed. UV-VIS spectrophotometers were used for spectral observation in both acidic and basic pH conditions. Different spectra from the individuals and 1:1, 1:2, and 2:1 complexes were obtained in the UV-VIS spectrophotometer. The drug complexes produced a distinct spectrum than its pure component. When Ranitidine hydrochloride is combined with Bisoprolol fumarate at a 1:1 ratio, the spectra suddenly alters as a result of interaction. The stability constant values at various levels of pH (1.2, 2.0, 3.0, 5.5, and 6.4) determined by the Ardon's plots are 0.896, 1.612, 1.200, 1.36, and 0.294. According to the stability constant values, Bisoprolol fumarate and Ranitidine hydrochloride interact in a moderately stable manner. Because of this, the complex displayed fluctuating activity during the test.

IIUC Studies, Vol.-20, Issue-1, June 2023, pp. 33-58