Evaluation of Rapid stool antigen test for the diagnosis of Helicobacter pylori infection in patients with dyspepsia

Authors

  • Salma Khatun Department of Microbiology, Ibrahim Medical College, Dhaka
  • Fahmida Rahman Department of Microbiology, Ibrahim Medical College, Dhaka
  • Khandaker Shadia Department of Microbiology, Ibrahim Medical College, Dhaka
  • Indrajit Kumar Dutta Department of Gastroenterology, Hepatobiliary and Pancreatic Diseases, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders, Dhaka
  • Mohammad Nazmul Hoq Department of Gastroenterology, Hepatobiliary and Pancreatic Diseases, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders, Dhaka
  • Farjana Akter Department of Microbiology, Ibrahim Medical College, Dhaka
  • Jalaluddin Ashraful Haq Department of Microbiology, Ibrahim Medical College, Dhaka

Abstract

Background and objectives: Several diagnostic assays are used for the detection of Helicobacter pylori infection in suspected peptic ulcer cases. H. pylori stool antigen test is a non-invasive method for the detection of active infection. The present study has evaluated the efficacy of rapid stool antigen test to diagnose H. pylori infection in patients with dyspepsia.

Materials and methods: Adult patients with complains of dyspepsia attending the Department of Gastroenterology, Hepatobiliary and Pancreatic Diseases (GHPD) of BIRDEM hospital for endoscopy were included. Gastric biopsy, blood and stool samples were obtained from each participant after informed written consent. Rapid urease test (RUT), serum H. pylori immunoglobulin A (IgA) and IgG and rapid H. pylori stool antigen (HpSAg) tests were performed. Only stool samples were obtained from 31 neonates aged 1 to 30 days as negative control for HpSAg test.

Results: A total of 91 adult patients with complain of dyspepsia were included in the study. Out of 91 cases, 17 (18.7%) and 74 (81.3%) had peptic ulcer and erosion respectively. HpSAg was positive in 63.7% cases compared to 42.9% and 62.6% respectively by RUT and IgA. The rate of HpSAg positivity was significantly higher (p<0.05) in ulcer compared to erosion cases. HpSAg test was positive in all (100%) RUT positive cases. Combination of HpSAg test and IgA yielded highest positive result in both ulcer (82.4%) and erosion (84%) cases. H. pylori IgG was positive in all cases.

Conclusion: The study has demonstrated that HpSAg test is an effective and non-invasive diagnostic tool to detect active H. pylori infection in suspected dyspeptic patients.

IMC J Med Sci 2016; 10(2): 39-44

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Published

2017-01-12

How to Cite

Khatun, S., Rahman, F., Shadia, K., Dutta, I. K., Hoq, M. N., Akter, F., & Haq, J. A. (2017). Evaluation of Rapid stool antigen test for the diagnosis of Helicobacter pylori infection in patients with dyspepsia. IMC Journal of Medical Science, 10(2), 39–44. Retrieved from https://banglajol.info/index.php/IMCJMS/article/view/31108

Issue

Vol. 10 No. 2 (2016)

Section

Original Articles

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