Comparative evaluation of rapid Salmonella Typhi IgM/IgG and Widal test for the diagnosis of enteric fever

Authors

  • Farjana Akter Department of Microbiology, Ibrahim Medical College, Dhaka, Bangladesh
  • Mahmuda Yeasmin 2Department of Virology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh
  • Md Zahangir Alam Bangladesh Specialized Hospital, Dhaka, Bangladesh
  • Md Rokibul Hasan Department of Microbiology, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM), Dhaka, Bangladesh
  • Fahmida Rahman 1Department of Microbiology, Ibrahim Medical College, Dhaka, Bangladesh
  • Elisha Khandker Department of Microbiology, Ibrahim Medical College, Dhaka, Bangladesh
  • Md Monirul Hoque Department of Microbiology, Ibrahim Medical College, Dhaka, Bangladesh
  • Lovely Barai Department of Microbiology, Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM), Dhaka, Bangladesh
  • - Md Mohiuddin Department of Microbiology, Ibrahim Medical College, Dhaka, Bangladesh
  • Md Shariful Alam Jilani Department of Microbiology, Ibrahim Medical College, Dhaka, Bangladesh

Keywords:

rapid Salmonella, enteric fever

Abstract

Background: Accurate and early diagnosis of enteric fever is a diagnostic challenge where facility for blood culture is not available. As a result, Widal test is still used widely in resource limited settings. Recently, user-friendly rapid immunochromatographic tests (ICT) have been introduced for quick diagnosis of enteric fever. So, we evaluated sensitivity and specificity of an immunochromatography based Salmonella Typhi IgM/IgG test kit and Widal test compared to blood culture for the diagnosis of enteric fever.

Method: The study was conducted in the Department of Microbiology, Ibrahim Medical College (IMC) and Bangladesh Institute of Research and Rehabilitation in Diabetes, Endocrine and Metabolic Disorders (BIRDEM) from June 2017 to September 2017. Clinically suspected enteric fever cases were included. Blood culture, Widal and Salmonella Typhi IgM/IgG detecting ICT were employed for the diagnosis of enteric fever.

Results: Out of 71 suspected cases of enteric fever, blood culture was positive in 36 cases (50.7%) while 42 (59.15%) and 35 (49.29%) cases were positive by Widal test and ICT respectively. Widal and ICT had sensitivity and specificity of 100% and 89.9% and 82.9% & 91.4% respectively.

Conclusion: Findings of the study suggest that both Widal and immunochromatographic tests can be used interchangeably for rapid diagnosis of enteric fever.

Ibrahim Med. Coll. J. 2020; 14(1): 18-25

Downloads

Download data is not yet available.
Abstract
1100
PDF
2415

Downloads

Published

2020-06-09

How to Cite

Akter, F., Yeasmin, M., Alam, M. Z., Hasan, M. R., Rahman, F., Khandker, E., Hoque, M. M., Barai, L., Md Mohiuddin, .-., & Jilani, M. S. A. (2020). Comparative evaluation of rapid Salmonella Typhi IgM/IgG and Widal test for the diagnosis of enteric fever. IMC Journal of Medical Science, 14(1), 18–25. Retrieved from https://banglajol.info/index.php/IMCJMS/article/view/47452

Issue

Vol. 14 No. 1 (2020)

Section

Original Articles

License

Authors who publish in IMCJMS agree to the following terms that:

  1. Authors retain copyright and grant IMCJMS the right of first publication of the work.

  2. Creative Commons Licence
    Articles in IMCJMS are licensed under a Creative Commons Attribution 4.0 International License CC BY-4.0.This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
  3. Authors are able to enter into separate, additional contractual arrangements for the distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  4. Authors are permitted to post their work online (e.g., in institutional repositories or on their website) as it can lead to productive exchanges, as well as greater citation of published work.