Detection and Quantification of HCV-RNA by RT-PCR among Anti-HCV Positive Patients

Authors

  • Wasila Rahman Assistant Professor of Microbiology, Armed Forces Medical College (AFMC), Dhaka, Bangladesh
  • - Md Rahimgir Ex-Professor and Head, Department of Microbiology, AFMC, Dhaka, Bangladesh
  • Arif Ahmed Khan Deputy Commandant, Armed Forces Institute of Pathology (AFIP), Dhaka, Bangladesh
  • Maj Suman Khisa Classified Specialist in Pathology, Border Guard Hospital, Thakurgaon, Bangladesh
  • Rahima Akter Officer on Special Duty, Director General of Health Services, Mohakhali, Dhaka, Bangladesh
  • Maj Imana Shahreen Trainee Officer, AFIP, Dhaka, Bangladesh

DOI:

https://doi.org/10.3329/jafmc.v15i1.48652

Keywords:

ELISA, HCV-RNA, Real time PCR

Abstract

Introduction: The most common contemporary strategy to diagnose chronic hepatitis C virus (HCV) infection consists of initial screening with an HCV enzyme immunoassay (EIA) antibody test followed by supplemental testing of positive screening tests with a quantitative HCV RNA assay to confirm the positive EIA and to determine whether they have active or resolved hepatitis C infection.

Objectives: To detect and quantify HCV-RNA by real-time polymerase chain reaction (real-time PCR) among anti-HCV positive patients and to identify the socio demographic factors among these patients.

Materials and Methods: This was a descriptive type of cross-sectional study which was conducted in Combined Military Hospital and Armed Forces Institute of Pathology, Dhaka cantonment. A total of 108 anti-HCV positive patients by enzyme-linked immunosorbent assay (ELISA), who were clinically suspected and advised for anti-HCV test, were selected randomly for the study and subjected to do HCV-RNA analysis during the period of October 2016 to September 2017.

Results: Out of 108 anti-HCV positive patients by ELISA, HCV-RNA was detected in 72 (66.7%) cases with mean value of HCV RNA quantification was 2013323.95±2695207.41 (IU/ ml). Majority of anti-HCV positive patients (29.6%) belonged to 51-60 years age group with male predominance (58.33%). It was observed that 43.52% patients came from middle income group family, 31.48% came from poor and 25.0% came from high income group family. Risk factor for HCV infected population was found maximum in dialysis patients (47.37%), followed by blood transfusion (13.89%), Injecting drug User (IDUs) (12.04%), surgery & intervention (9.26%) and sexual transmission (1.85%). Mean alanine aminotransferase (ALT) was found 67.30±44.99 U/L among HCV-RNA detected patients (p< 0.05).

Conclusion: The quantification of HCV RNA by RT-PCR will be helpful to rationalize the treatment, enhance antiviral responses and mitigate mortalities of HCV infected patients.

Journal of Armed Forces Medical College Bangladesh Vol.15 (1) 2019: 84-86

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Published

2020-08-20

How to Cite

Rahman, W., Md Rahimgir, .-., Khan, A. A., Khisa, M. S., Akter, R., & Shahreen, M. I. (2020). Detection and Quantification of HCV-RNA by RT-PCR among Anti-HCV Positive Patients. Journal of Armed Forces Medical College, Bangladesh, 15(1), 84–86. https://doi.org/10.3329/jafmc.v15i1.48652

Issue

Vol. 15 No. 1 (2019)

Section

Original Papers

License

Submission of a manuscript for publication implies the transfer of the copyright from the author to the publisher upon acceptance. Accepted manuscripts become the permanent property of the Journal of Armed Forces Medical College and may not be reproduced by any means in whole or in part without the written consent of the publisher.