Histological Variation in the Number and Diameter of the Postmortem Human Pancreatic Islets in Relation to Age and Sex: Insight from North East Bangladesh

Authors

  • Anup Kumar Biswas Classified Specialist Anatomy and Clinical Embryologist, CMH, Dhaka
  • Mytrayee Dev Roy Former Associate Professor, Department of Pharmacology, Jalalabad Ragib Rabeya Medical College, Sylhet
  • Md Ashraful Islam Chowdhury Associate Professor of Anatomy, Armed Forces Medical College, Dhaka
  • Mohammad Nessar Uddin Ahmed Associate Professor of Physiology, Armed Forces Medical College Dhaka
  • Ayesha Akter Toshi Lecturer, Armed Forces Medical College, Dhaka
  • Raju Chowdhury Assistant Professor of Anatomy, North East Medical College, Sylhet.

DOI:

https://doi.org/10.3329/jafmc.v20i1.73851

Keywords:

Pancreas, Islets, Cadaver, Ocular micrometer.

Abstract

Background: Disease pattern as well as diagnostic and treatment options may be helped by examining the number and diameter of Islets of human pancreas, as the change with age. It seems that there is a research vacuum in this area and demands more studies, including gross anatomical studies, with data available in Bangladesh.

Objective: To find out the histological variations of post mortem human pancreas in relation to age and sex of north east region of Bangladesh.

Methods: Fifty human postmortem pancreases were selected. The inclusion criteria was dead bodies autopsied within 36 hours of death; while considerable signs of decomposition, decomposed dead body, dead body autopsied exceeds 36 hours after death, presence of gross pancreatic disease and poisoning cases were excluded. The obtained specimens of pancreas were classified according to the age and sex. Simultaneously, the different parts of the pancreas (head, neck, body and tail) were separated. Then each part of the gland was placed in a tray and sectioned transversely. From these slices, small pieces of tissue were taken for histological procedure. Then the tissues were processed and stained with haematoxylin and Eosin (H&E) stain. The slides were examined under microscope in low and high magnification. Number of the Islets per mm2 area and the average transvertical diameter of the Islets of Langerhans were measured with ocular micrometer.

Results: The mean number of Islets (per mm2) of the pancreas was 1.91 (SD±0.81) and diameter of Islets of the pancreas was 153.44μm (SD±7.22). The mean number of Islets (per mm2) was 2.05 (SD±0.69); 2.31 (SD±0.96) and 1.38 (SD±0.55) in the age group of 5 to 20 years, 21 to 40 years and years 41 to 64 years respectively (p=0.124). The mean number of Islets (per mm2) was 1.78 (SD±0.71) in male and 2.08 (SD±0.94) in female (p=0.449). The mean diameter of Islets was 151.0 μm (SD±5.9); 156.7 μm (SD±10.3) and 152.7 μm (SD±4.1) in the age group of 5 to 20 years, 21 to 40 years and years 41 to 64 years respectively (p=0.401). The mean diameter of Islets was 158.7μm (SD±5.3) in male and 146.9μm (SD±1.36) in female (p<0.01). A negative correlation (non-significant) was observed between age and number of Islets per mm2 (p=0.506). A positive correlation (non-significant) was observed between age and diameter of Islets (p=0.861).

Conclusion: Age related changes were found in the diameter of pancreatic Islets. Most of the pancreatic disease can be managed with conservation of pancreas by invasive and non-invasive method. Detail knowledge on pancreatic Islets will help the physician to manage pancreatic disease.

JAFMC Bangladesh, Vol 20, No 1 (June) 2024: 26-29

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Published

2024-06-30

How to Cite

Biswas, A. K., Roy, M. D. ., Chowdhury, M. A. I. ., Ahmed, M. N. U. ., Toshi, A. A. ., & Chowdhury, R. (2024). Histological Variation in the Number and Diameter of the Postmortem Human Pancreatic Islets in Relation to Age and Sex: Insight from North East Bangladesh. Journal of Armed Forces Medical College, Bangladesh, 20(1), 26–29. https://doi.org/10.3329/jafmc.v20i1.73851

Issue

Vol. 20 No. 1 (2024)

Section

Original Papers

License

Submission of a manuscript for publication implies the transfer of the copyright from the author to the publisher upon acceptance. Accepted manuscripts become the permanent property of the Journal of Armed Forces Medical College and may not be reproduced by any means in whole or in part without the written consent of the publisher.