@article{Jawasreh_Ismail_2019, title={Effects of calpastatin gene polymorphism on hematology and selected serum biochemical parameters in Awassi lambs}, volume={6}, url={https://banglajol.info/index.php/JAVAR/article/view/41758}, abstractNote={<p><strong>Objective: </strong>This study was conducted to investigate the effect of <em>Msp</em>I polymorphism in the calpastatin (CAST) gene on hematology and selected serum biochemical parameters in Awassi lambs.</p> <p><strong>Materials and Methods: </strong>CAST genotypes of 31 Awassi lambs were determined using polymerase chain reaction—restricted fragment length polymorphism method. Hematology, serum biochem­ical analyses, serum levels of triiodothyronine, thyroxine, and cortisol were determined using rou­tine laboratory procedures.</p> <p><strong>Results: </strong>Two CAST genotypes were detected with frequencies of 0.65 and 0.35 for MN (three major bands of 622, 336, and 268 bp) and NN (two major bands of 336 and 268 bp), respectively. Allele frequencies were 0.49 and 0.51 for M and N alleles, respectively. Animals with MN <em>MspI </em>CAST genotype had significantly (<em>p </em>< 0.05) higher neutrophil percentage and neutrophil to lymphocyte ratio but, significantly (<em>p </em>< 0.05) lower lymphocyte percentage and neutrophil to lymphocyte ratio than NN <em>MspI </em>CAST genotype. Serum T3 and cortisol concentrations were significantly (<em>p </em>< 0.05) higher in MN <em>MspI </em>CAST genotype than the NN <em>MspI </em>CAST genotype.</p> <p><strong>Conclusion: </strong>Results of this study indicate that CAST gene heterozygous individuals are healthier than homozygous individual, which may explain the superiority of the CAST gene heterozygous animals in growth performance.</p> <p>J. Adv. Vet. Anim. Res., 6(2): 193-196, June 2019</p>}, number={2}, journal={Journal of Advanced Veterinary and Animal Research}, author={Jawasreh, Khaleel Ibrahem and Ismail, Zuhair Bani}, year={2019}, month={Jun.}, pages={193–196} }