TY - JOUR AU - Islam, Md Ashraful AU - Biswas, Proteek AU - Sabuj, Abdullah Al Momen AU - Haque, Zobayda Farzana AU - Saha, Chayan Kumer AU - Alam, Md Monjurul AU - Rahman, Md Tanvir AU - Saha, Sukumar PY - 2019/09/12 Y2 - 2024/03/28 TI - Microbial load in bio-slurry from different biogas plants in Bangladesh JF - Journal of Advanced Veterinary and Animal Research JA - J. Adv. Vet. Anim. Res VL - 6 IS - 3 SE - Original Articles DO - UR - https://banglajol.info/index.php/JAVAR/article/view/43114 SP - 376-383 AB - <p><strong>Objective: </strong>The study was aimed to isolate, identify, and characterize common indicator bacteria, including <em>Escherichia coli</em>, <em>Salmonella </em>spp<em>.</em>, and <em>Staphylococcus </em>spp<em>. </em>in manure and bio-slurry sam­ples of different livestock farms and biogas plants of Bangladesh.</p><p><strong>Materials and Methods: </strong>A total of 114 samples of manure and bio-slurry were collected from different livestock farms and biogas plants in Bangladesh. The total viable count (TVC), <em>E. coli, Salmonella </em>spp., and <em>Staphylococcus </em>spp. counts were determined by the spread plate technique method. Isolation and identification were performed by colony characteristics, staining, bio­chemical tests, and, finally, by using PCR. Antibiotic susceptibility test of the isolated bacteria was tested against commonly used antibiotics by using the disk diffusion method.</p><p><strong>Results: </strong>The mean TVC, <em>E. coli</em>, <em>Salmonella </em>spp., and <em>Staphylococcus </em>spp. counts were ranged from 8.19–10.75, 5.2–6.96, 5.81–6.87, 5.68–7.68 in manure samples and 7.26–8.65, 3.82–5.2, 4–5.54, 3.14–5.9 log cfu/gm in bio-slurry, respectively. In anaerobic digester after 30 days digestion, the presence of <em>E. coli, Salmonella </em>spp., and <em>Staphylococcus </em>spp. varied from 0–5.11, 0–4.84, and 0–5.59 log cfu/gm at 25°C, 27°C, 29°C, and 45°C temperature. Above-mentioned bacteria were absent in bio-slurry collected from anaerobic digester after 60 days digestion at environmental temperature. Bacterial counts were reduced significantly in both household slurry pits and exper­imental anaerobic digester. Antibiotic susceptibility results revealed that multidrug-resistant indi­cator bacteria were present in the bio-slurry samples.</p><p><strong>Conclusion: </strong>Our findings conclude that the microbial load after treatment of animal manure via anaerobic digestion (Biogas plant) was grossly reduced and the reduction of bacterial pathogen depends on the duration and temperature of digestion.</p><p>J. Adv. Vet. Anim. Res., 6(3): 376-383, September 2019</p> ER -