Foreground selection through SSRs markers for the development of salt tolerant rice variety

Abstract

Salinity is a great problem for rice production worldwide incurring substantial yield loss; a great threat towards food security. Marker-assisted backcrossing is one of the feasible methods to develop a new salt tolerant rice cultivar to cope with the challenge. The study was focused on to introgress salt tolerant genes from a tolerant rice line, FL-478 to Binadhan-7, an early, agronomically suitable and susceptible variety. Backcrossing was done during boro season; where Binadhan-7 was the recurrent parent and FL-478 was the donor parent. 141 BC₁F₁ lines were developed, which were subjected to foreground selection; the first level of selection of marker assisted backcrossing program. The aim of foreground selection was to identify the introgressed lines. 141 BC₁F₁ populations were evaluated with tightly linked salt tolerant markers; AP3206f, RM3412b and RM336. A total of 47 heterozygous BC₁F₁ lines were selected finally, which have alleles of both of the parents. Those introgressed lines could be efficiently used in further development of a stable early salt tolerant rice variety.

DOI: http://dx.doi.org/10.3329/jbau.v11i1.18215

J. Bangladesh Agril. Univ. 11(1): 67-72, 2013