Effects of probiotic and Vitamin C on domestication and breeding  performance of indigenous koi, Anabas testudineus under cage system

Md Kamal Hossain; A K Shakur Ahammad; Md Borhan Uddin Ahmed; Md Fazla Rabbi; Md Ashraful  Haque; Biraj Kumar Datta

Authors

Md Kamal Hossain Department of Fisheries Biology & Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
A K Shakur Ahammad Department of Fisheries Biology & Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
Md Borhan Uddin Ahmed Department of Fisheries Biology & Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
Md Fazla Rabbi Department of Fisheries Biology & Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
Md Ashraful Haque Department of Fisheries Biology & Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
Biraj Kumar Datta Department of Fisheries Biology & Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

Keywords:

Indigenous climbing perch, Domestication, Growth performance, GSI, induced breeding, Pituitary gland extract

Abstract

An experiment was accomplished on domestication and induced breeding of indigenous climbing perch, Anabas testudineus. For domestication, fish were stocked and reared for six months in nine cages (each having 1.0 m³ in size) set in three ponds (Pond 1, 2 and 3) so that three cages were set up in each pond. Three supplementary feeds such as feed mixed with vitamin C, only supplementary feed and supplementary feed with probiotic (Bacilus subtilis and Aerobacter sp.) considered as treatment T₁, T₂ and T₃, respectively were provided to triplicate cages in each pond and the cages were considered as replications R₁, R₂, R₃. Highest growth in terms of net length and weight gain was found in T₃ (9.35±2.65 cm and 44.58±4.68 g) followed by T₁ (9.00±2.03 cm and 37.62±3.45 g), and T₂ (6.18±1.57 cm and 34.24±3.08 g), respectively. Likewise, the highest average value of GSI was found in T₃ (7.26±2.12) in the month of April while lowest average value of GSI (0.64±0.21) was found in T₂ in the month of July. Three induced breeding trials were conducted using carp pituitary gland extract (PG) where female broods received 6-8 mgkg^-1 body weight and males received 2-4 mg kg^-1 body weight. Fish in T₃ injected with PG 4 mgkg^-1 for male and 8 mgkg^-1 for female showed highest result in case of ovulation rate (96.67±5.77), fertilization rate (75.75±2.00%) and hatching rate (90.25±2.18%) followed by T₂ (ovulation, 80±10%, fertilization, 70.25±2.22%, hatching and 85.65±1.80%) and T₁ (ovulation, 73.33±5.77, fertilization, 66.23±1.56% and hatching, 72.75±2.06%).The survival rate of fry was highest in T₃ (65.9±1.09%) followed by T₂ (60.75±2.00%) and T₁ (55.5±2.46%). It was observed that, the mixture of probiotic with feed was more suitable for higher growth and GSI. 2.0 g probiotic (VC-7, Team aqua corporation, Taiwan) and 20 g molasses for 5 L of water were found better result for induced breeding of A. testudineus.