Determination of genetic variations among different <i>Trichoderma</i> isolates using RAPD marker in Bangladesh

Authors

  • MSI Sagar Department of Biotechnology, Bangladesh Agricultural University, Mymensingh
  • MB Meah Department of Plant Pathology, Bangladesh Agricultural University Mymensingh
  • MM Rahman Department of Biotechnology, Bangladesh Agricultural University, Mymensingh
  • AK Ghose Department of Biotechnology, Bangladesh Agricultural University, Mymensingh

DOI:

https://doi.org/10.3329/jbau.v9i1.8738

Keywords:

Genetic diversity, Trichoderma, RAPD

Abstract

Some Trichoderma isolates were collected from different locations of Bangladesh for evaluating their bioefficiency by determining their genetic variations. PCR-based Random Amplified Polymorphic DNA (RAPD) Marker employing 3 decamer primers produced 29 scorable bands of which all (100%) were polymorphic. The co-efficient of gene differentiation (Gst) was 1.0000 reflecting the existence of high level of genetic diversity among the isolates. The Unweighted Pair Group Method of Arithmetic Means (UPGMA) dendrogram constructed from Nei’s (1972) genetic distance produced 2 main clusters (16 isolates in cluster 1 and 19 isolates in cluster 2). The result indicating their genetic diversity has opened new possibility of using the most efficient and more isolates of Trichoderma in the preparation of effective biopesticide.

Keywords: Genetic diversity; Trichoderma; RAPD

DOI: http://dx.doi.org/10.3329/jbau.v9i1.8738

JBAU 2011; 9(1): 9-20

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How to Cite

Sagar, M., Meah, M., Rahman, M., & Ghose, A. (2011). Determination of genetic variations among different <i>Trichoderma</i> isolates using RAPD marker in Bangladesh. Journal of the Bangladesh Agricultural University, 9(1), 9–20. https://doi.org/10.3329/jbau.v9i1.8738

Issue

Vol. 9 No. 1 (2011)

Section

Crop Science

License

© 2003-2017 Bangladesh Agricultural University Research System.

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Journal of the Bangladesh Agricultural University is licensed under a Creative Commons Attribution 4.0 International License.

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