@article{Bhadra_Obaidullah_Sultana_Ahmed_Islam_2019, title={Genetic diversity analysis in cashew (Anacardium occidentale L.) germplasm using RAPD marker: Genetic distance in germplasm using RAPD}, volume={17}, url={https://banglajol.info/index.php/JBAU/article/view/44606}, abstractNote={<p><em>Anacardium occidentale</em> L., commonly known as cashew nut, belongs to the family Anacardiaceae.  It is regarded as a high valued fruit nut crop world-wide. Potential of this economically important nut  is under-utilized in Bangladesh in spite of having all favorable agro-climatic conditions. The objective of the present investigation was to characterize six cashew accessions using Random Amplified Polymorphic DNA (RAPD) markers. Four random primers <em>viz.</em> OPE-02, OPE-18, OPK-03 and OPB-15 were used to amplify DNA segments. A total of 33 reproducible bands were obtained, out of which 11 were monomorphic and 22 were polymorphic. On average 74.12% polymorphism was observed. . Primers OPB-15 and OPK-03 yielded 100% polymorphism and OPE 02 and OPE 18 produced 33.33% and 63.16 % polymorphism, respectively. Cluster analysis revealed two main distinct groups, first group included GP-1 and the second consisted of five genotypes <em>viz.</em> GP-2, GP-3, GP-4, GP-5and GP-6 The major cluster- II was further subdivided into two minor clusters i.e. minor cluster- III and IV. Minor cluster- III contained only one genotype GP-4. Minor cluster- IV consists of four rest genotypes. The genetic distance between the groups was found low and varied from 0.002 to 0.0308. Maximum genetic distance was observed between GP-1 and GP-2cashew germplasm and minimum between GP-5 and GP-6. The low genetic distance which is unusual for this out crossing long-lived tree species, indicates the probability of having common ancestry among the germplasm or may be due to the use of a narrow range of populations for the investigation.</p> <p><!--StartFragment --></p> <p><span style="font-size: 9px;"><strong><span style="font-family: ’Times New Roman’, Italic; color: #000000;">J Bangladesh Agril Univ 17(4): 461–465, 2019</span></strong></span></p>}, number={4}, journal={Journal of the Bangladesh Agricultural University}, author={Bhadra, T and Obaidullah, AZM and Sultana, Mst Sabiha and Ahmed, M and Islam, MM}, year={2019}, month={Dec.}, pages={461–465} }