TY - JOUR AU - Jewel, MAS AU - Rahman, MM AU - Islam, MN PY - 2007/11/06 Y2 - 2024/03/28 TI - Study of Genetic Variation in Different Hatchery Populations of Common Carp (Cyprinus carpio) of Mymensingh District in Bangladesh using Microsatellite DNA Markers JF - Journal of Bio-Science JA - J bio-sci. VL - 14 IS - 0 SE - Articles DO - 10.3329/jbs.v14i0.454 UR - https://banglajol.info/index.php/JBS/article/view/454 SP - 113-120 AB - Genetic variation of two strains (Scaled and Mirror) of six hatchery populations of common carp (<i>Cyprinus carpio</i>) was studied using three microsatellite loci (<i>MFW13</i>, <i>MFW17</i> and <i>MFW28</i>). The loci were amplified by Polymerase Chain Reaction (PCR). The PCR products were electrophoresed on 6% polyacrylamide gel and the alleles were visualized by silver nitrate staining. All the three loci were found polymorphic. The average number of alleles was the highest in the population *SC-SMK (5.00) and lowest in **MC-SMK (4.00). Total number of null allele was more in mirror carp populations (6) than scaled carp populations (4). The average observed heterozygosity (<i>Ho</i>) ranged from 0.54 to 0.67. The average observed heterozygosity (<i>Ho</i>) was lower than the average expected heterozygosity (<i>He</i>) in case of all the populations. The population ***SC-BCH had high level of genetic variation in comparison to other five populations because this population had negative 1-<i>Ho/He</i> values in case of two loci out of three analyzed. The rest five populations had negative 1-<i>Ho/He</i> values only in one locus. In 6 of a total of 18 tests, significant deviations from Hardy-Weinberg expectations (HWE) were detected. Pairwise <i>Fst</i> values were used to detect the genetic distance between the pairs of populations. The <i>Fst</i> value was highest (0.153) between the population MC-AFF and MC-SMK. Nei’s genetic distance value was also highest (0.462) between these populations. The <i>Fst</i> value of 0.153 represents a high level of population differentiation. The lowest <i>Fst</i> value (0.001) was found between SC-SMK and MC-SMK. This value was statistically insignificant. *SC–SMK- scaled carp from Sagor Matsho Khamar **MC – SMK- mirror carp from Sagor Matsho Khamar ***SC-BCH- scaled carp from Brahmaputra Char Fishery <b>Key words:</b> Genetic variation, common carp, Microsatellite DNA markers J. bio-sci. 14: 113-120, 2006 ER -