GeneXpert MTB/RIF Assay for Rapid Identification of Mycobacterium Tuberculosis and Rifampicin Resistance Directly from Sputum Sample

Authors

  • Nourjahan Laskar Assistant Professor, Department of Microbiology, International Medical College, Gazipur
  • Md Akram Hossain Professor, Department of Microbiology, Mymensingh Medical College, Mymensingh
  • Jannatul Fardows Assistant Professor, Department of Microbiology, Kumudini Women?s Medical College, Tangail
  • Mominur Rahman Registrar, Department of Neuroanaesthesia and Neuro ICU, Apollo Hospitals, Dhaka

DOI:

https://doi.org/10.3329/jemc.v7i2.32653

Keywords:

GeneXpert assay, MDR TB, Sensitivity, Specificity

Abstract

Background: The World Health Organization has endorsed the use of molecular methods for the detection of tuberculosis (TB) and drug resistant TB as a rapid method. In Bangladesh, the Xpert MTB/RIF assay has been implemented into reference laboratories for diagnosis of TB and also MDR TB.

Objective: Drug resistant tuberculosis has long been a common problem prevailing in our country. The present study focused on the rapid identification of Mycobacterium tuberculosis as well as drug resistance.

Materials and Methods: Sputum samples from a total of 107 cases, assumed as multi-drug resistance tuberculosis, were studied through GeneXpert assay.

Results: Out of 107 cases, 91 (85.05%) were detected having M. tuberculosis ? 64 (59.81%) were rifampicin sensitive and 27 (25.23%) were rifampicin resistant. The sensitivity and specificity of the GeneXpert are 87.64% and 75% respectively.

Conclusion: GeneXpert assay can be considered for the rapid diagnosis of drug resistant tuberculosis.

J Enam Med Col 2017; 7(2): 86-89

Downloads

Download data is not yet available.
Abstract
3
PDF
5

Author Biography

Nourjahan Laskar, Assistant Professor, Department of Microbiology, International Medical College, Gazipur



Downloads

Published

2017-06-04

How to Cite

Laskar, N., Hossain, M. A., Fardows, J., & Rahman, M. (2017). GeneXpert MTB/RIF Assay for Rapid Identification of Mycobacterium Tuberculosis and Rifampicin Resistance Directly from Sputum Sample. Journal of Enam Medical College, 7(2), 86–89. https://doi.org/10.3329/jemc.v7i2.32653

Issue

Vol. 7 No. 2 (2017)

Section

Original Articles

License

  • Copyright on any research article is transferred in full to Journal of Enam Medical College upon publication in the journal. The copyright transfer includes the right to reproduce and distribute the article in any form of reproduction (printing, electronic media or any other form).
  • Articles in the Journal of Enam Medical College are Open Access articles published under the Creative Commons CC BY License (https://creativecommons.org/licenses/by/4.0/)
  • This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.