Detection of Extended Spectrum Beta-lactamase (ESBL) Producing Gram Negative Bacteria from Different Clinical Specimens of Largest Teaching Hospital in Dhaka city of Bangladesh

Abstract

Background: Detection of Extended spectrum beta lactamase (ESBL) enzyme producing bacteria in hospital settings is very important as ESBL genes are transmissible.

Objective: This study was carried out to determine the distribution of ESBL producing gram negative isolates at a tertiary care hospital in Dhaka city which deals with different types of patients.

Methodology: Clinical specimens were collected from the patients attending the microbiology laboratory of Dhaka Medical College from outpatient and inpatient department, Dhaka, during the period of July 2014 to June 2015.

Results: Out of 191-gram negative bacteria 73 (38.22%) were positive for ESBL production by DDS test. Those out of 73 ESBL producers 50 (68.49%) were positive for ESBL encoding gene blaCTX-M-15 and 36 (49.32%) for blaOXA-1 by PCR. By DDS test, Among the ESBL producers, Escherichia coli was the highest (43.84%%) which was followed by Pseudomonas species (15.07%), Klebsiella species (15.07%), Citrobacter spp. (8.22%) and Proteus species (6.85%). Out of 24 Esch. coli isolated from outpatient department, 7(29.2%) were positive for ESBL. On the other hand, out of 30 Esch coli isolated from inpatient department, 25 (83.33%) were positive for ESBL. The difference was statistically significant (p<0.001).

Conclusion: In conclusion, the distribution of ESBL producers is more among the hospitalized patients than the patients of the community.

Journal of Monno Medical College, June, 2025; 11(1) : 49-53