Antimicrobial and Cytotoxic Activities of the Crude Extracts and Isolated Compounds of <i>Xylocarpus mollucensis</i>

Abstract

The fractionated crude extracts and three isolated pure compounds XM-1, XM-2 and XM-3 from stem bark of Xylocarpus mollucensis were screened for their antibacterial and antifungal activities and cytotoxicity against brine shrimp nauplii. Petroleum ether, ethyl acetate (EtOAc) and methanol (MeOH) extracts and the compounds isolated from EtOAc fractions were studied for their antimicrobial activities. Cytotoxic activities were conducted only with EtOAc extract and its selected fractions. The EtOAc extract showed promising antimicrobial activities against all the gram positive and gram negative bacteria whereas petroleum ether extract showed moderate activities and the MeOH extract did not show any antimicrobial activities. The isolated pure compounds XM-1, XM-2 and XM-3, whose structures were not elucidated, exhibited activities against most of the bacterial strains. The cytotoxicity towards brine shrimp nauplii of the crude EtOAC extract and its selected fractions were studied. The LC₅₀ values of the EtoAc extract was 12.6 ?g/ml and for the fractions 2, 5, 8 and 13 were 17.78, 13.34, 14.13 and 15.85 ?g/ml, respectively. Key words: Antimicrobial; Cytotoxic; Xylocarpus; Meliaceae. Dhaka Univ. J. Pharm. Sci. 6(2): 109-112, 2007 (December)