TY - JOUR AU - Hossain, M Shakhawoat AU - Hasan, MK AU - Shoeb, M AU - Mamun, M I R AU - Nahar, N AU - Ali, L AU - Mosihuzzaman, M AU - Rokeya, B AU - Chaudhury, HS PY - 2020/12/10 Y2 - 2024/03/28 TI - Antidiabetic Activity of Momordica charantia L. and Mechanism of Insulin Secretion of 1-Butanol Soluble Part on Type 2 Diabetic Model Rats JF - Dhaka University Journal of Pharmaceutical Sciences JA - Dhaka Univ. J. Pharm. Sci VL - 19 IS - 2 SE - Articles DO - 10.3329/dujps.v19i2.50625 UR - https://banglajol.info/index.php/JPharma/article/view/50625 SP - 111-117 AB - <p>The <em>in vivo </em>effects of fruit pulp juice (MC-PJ) of <em>Momordica charantia </em>and its 1-butanol soluble part (MC-BP) and aqueous soluble part (MC-AP) on blood glucose of type 2 diabetic rats were studied. <em>In vitro </em>insulin secretion in response to MC-BP and MC-AP from whole perfused pancreas was measured. For elucidating the mechanism of insulinotropic action, the insulin secretory activity of MC-BP in the presence of 11 mM glucose, 50 μM verapamil (Ca++ channel blocker), 8 mM diazoxide (K+ ATP channel opener) and 10 mM theophylline (cAMP phosphodiesterase inhibitor) were studied. Serum glucose was measured by glucose oxidase-peroxidase method and rat insulin was assayed by specific ELISA. In the <em>in vivo </em>study, MC-BP significantly opposed the rise of serum glucose compared to control at 105 min (p&lt;0.05). Although the MC-AP and MC-PJ lowered the serum glucose both at 60 and 105 min, these were not statistically significant. In the <em>in vitro </em>study, only MC-BP produced 22-fold increase in insulin secretion from the perfused pancreas at nonstimulatory glucose level, which was significant (basal vs. MC-BP, 0.071±0.009 vs. 1.563±0.150 ng/ml, p&lt;0.001). The MC-BP also enhanced the insulin secretion from the glucose-stimulated pancreas (p&lt;0.001). The MC-BP induced insulin secretion was not affected in presence of diazoxide and verapamil. The obtained results also showed that MC-BP enhanced the insulin secretory effect of theophylline (p&lt;0.001). The findings indicate that MC-BP has stimulatory effects on physiological pathways of insulin secretion which may underlie its reported antidiabetic action.</p><p>Dhaka Univ. J. Pharm. Sci. 19(2): 111-117, 2020 (December)</p> ER -