Lethal Effects of Salmonella Toxin Isolated from Layer Chickens

Abstract

This study was conducted to determine lethal effects of Salmonella toxin isolated from layer chickens. The chickens were collected from Paragon Poultry Farm, Rangpur and Ahana Poultry Farm, Dinajpur and brought to the Microbiology Laboratory at the Hajee Mohammad Danesh Science and Technology University, Dinajpur, to isolate and identify Salmonella spp., and subsequent toxin separation from the isolated organisms for the detection of lethal effects of the toxin. The lethality of isolated toxin was evaluated in day-old-chick. The toxicity in case of oral administration was 50%, whereas the rate was 80% when chicks were administered through intra-peritoneal route. The chick mortality rate was 65%. For toxin extraction, Salmonellae were isolated and identified from layer chickens. Out of 196 organ samples, 51 were found to be positive for Salmonella spp., of which 33 and 18 were of Paragon Poultry Farm and Ahana Poultry Farm, respectively. Total 60 chickens (27 pullets and 33 layers) were screened. The percentages of positive samples (liver, spleen, lung, heart) from these farms were 29.82 and 20.73%, respectively and the average prevalence of Salmonellae was 26.02%. In case of Paragon Poultry Farm, the prevalence of salmonellosis in pullet was 23.08% and in layer, it was 35.48%, whereas in case of Ahana Poultry Farm, the prevalence was 16.67 and 23.91%, respectively.

DOI: http://dx.doi.org/10.3329/mh.v1i1.13708

Microbes and Health Vol.1(1) June 2012 pp.19-22