Isolation and Characterization of Microflora from Human Urine

Authors

  • Kohinoor Parvin Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202
  • Nahid Rahman Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202
  • M Mostafizur Rahman Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202
  • M Alimul Islam Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202

DOI:

https://doi.org/10.3329/mh.v2i1.17257

Keywords:

Bacterial flora, urinary tract infection (UTI), isolation and antibiotic sensitivity profile

Abstract

The research work was conducted to isolate & characterized the bacteria isolated from mid-stream urine of human. A total of 30 samples were collected from patients with urinary tract infection (n=10samples); diabetic patients (n=10samples) & apparently healthy individuals (n=10samples). Escherichia coli, Staphylococcus spp. and Bacillus spp. was the predominant bacterial flora of human urine. Among the isolates, the prevalence of Escherichia coli was highest (80%) compared to Staphylococcus spp.(14%) and Bacillus spp.(6%). E. coli isolated from 6 different sources were found to be highly virulent, moderately virulent, less virulent and avirulent categories as observed in day-old suckling mice. Antibiotic sensitivity profiles suggest that nalidixic acid will be the first drugs of choice to treat the UTI caused by E. coli and arythromycin, ampicillin and azithromycin will be the second drugs of choice to treat the UTI caused by Staphylococcus spp and Bacillus spp. respectively.

Microbes and Health, June 2013, 2(1): 15-18

DOI: http://dx.doi.org/10.3329/mh.v2i1.17257

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Published

2014-02-24

How to Cite

Parvin, K., Rahman, N., Rahman, M. M., & Islam, M. A. (2014). Isolation and Characterization of Microflora from Human Urine. Microbes and Health, 2(1), 15–18. https://doi.org/10.3329/mh.v2i1.17257

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Articles