TY - JOUR AU - Parvin, S. AU - Kausar, M. AU - Haque, M. Enamul AU - Khalekuzzaman, M. AU - Sikdar, B. AU - Islam, M. Asadul PY - 2015/01/15 Y2 - 2024/03/29 TI - In vitro propagation of muskmelon (Cucumis melo L.) from nodal segments, shoot tips and cotyledonary nodes JF - Rajshahi University Journal of Life & Earth and Agricultural Sciences JA - Rajshahi Univ. j. life earth agric. sci. VL - 41 IS - 0 SE - Articles DO - 10.3329/rujleas.v41i0.21627 UR - https://banglajol.info/index.php/RUJLEAS/article/view/21627 SP - 71-77 AB - A rapid and efficient protocol is outlined for in vitro propagation of muskmelon<br />(Cucumis melo L.) Shoot tips, nodal segments and cotyledonary nodes from in<br />vitro grown seedlings were used as explants. The explants were inoculated on MS medium fortified with different combinations and concentrations of growth<br />regulators viz., BAP, NAA, GA3 and IBA for multiple shoot regeneration.<br />Effective result was found on MS medium supplemented with 2.0 mg/l BAP, in<br />which 90% and 70% cultures induced multiple shoots from nodal segments and<br />shoot tip explants, respectively. Whereas, 70% cultures of cotyledonary nodes<br />were found to induced shoots on MS medium with 1.5 mg/l BAP + 0.1 mg/l GA3. In vitro regenerated shoots were subcultured on half strength MS medium<br />supplemented with different concentrations of IBA and NAA for successful root<br />induction and the effective result (up to 70%) was found in medium with 1 mg/l<br />IBA. Well rooted in vitro grown plantlets were acclimatized in sandy soil, where<br />as 70% plantlets survived ER -