Stamford Journal of Microbiology 2019-02-03T06:30:23+00:00 Dr. Rashed Noor Open Journal Systems Published by the Department of Microbiology, Stamford University Bangladesh. Full text articles available. Microbiological quality analysis of fresh vended fruit juices and water sold in roadside stalls in Dhaka Metropolis by MPN method 2019-02-03T06:30:05+00:00 Mahamuda Akther Eva Shawda Shafiq Shreya Tasnia Ahmed <p>Quality of drinking water and juice is very important because if the quality deteriorates by the contamination of faecally originated microorganisms, it may cause serious diarrhea associated problems leading to death. In overpopulated countries like Bangladesh, this is a common scenario to experience diarrheal diseases due to drinking non-potable water as well as contaminated fresh juices. Present study was conducted to determine the quality of drinking water and juice by detection of indicator bacteria <em>Escherichia coli </em>by MPN (Most Probable Number) method which was performed by three consecutive steps including presumptive test, confirmed test and completed test. Other gram negative bacteria were also identified by biochemical methods. The indicator bacterium <em>Escherichia coli </em>was detected in two water samples out of 15 samples and one juice sample out of fifteen samples respectively during the MPN test method. Other Gram negative bacteria found in both water and juice samples included <em>Klebsiella </em>spp., <em>Alcaligenes </em>spp., Pseudomonas spp. and <em>Proteus </em>spp. The quality of drinking water and juice was found to be good in Bangladesh but proper hygiene should be maintained more strictly to avoid the contamination by other gram negative bacteria which are also capable of causing disease.</p> <p>Stamford Journal of Microbiology, Vol.7(1) 2017: 1-6</p> 2017-01-31T00:00:00+00:00 ##submission.copyrightStatement## Study of antimicrobial activity of two common anti-cough formulations sold in Bangladesh 2019-02-03T06:30:07+00:00 Tahmina Shammi Md Ismam Khalid <p>Coughing is a physiological process of clarifying the mucosal obstruction through the airways is often be complicated by the invasion following infection by an array of microorganisms. A number of anti-cough formulations are available to combat coughing which is a non-antibiotic drug by nature; however, in some instances these drugs may exhibit anti-microbial trait in action probably due to the chemical properties of the contents. Present study aimed to determine such anti-microbial activity of two commonly used anti-cough mixtures which are locally formulated by the reputed pharmaceuticals in Bangladesh. The antibiotic susceptibility pattern of five laboratory strains of <em>Escherichia coli, Klebsiella sp., Bacillus cereus, Pseudomonas aeruginosa and Staphylococcus aureus </em>were tested by the agar well diffusion method against these two oral liquid samples under investigation used as anti-cough drugs. Sample 1 was found to be highly effective against all of the five test organisms whereas sample 2 was found to be effective against four of the tested organisms. Interestingly, even the lowest amount (50 μl) of both drugs were able to mount inhibitory action against at least two test organisms, the highest amount of both drugs have been found to pose more antimicrobial action than their positive control Gentamycin 10 mg.</p> <p>Stamford Journal of Microbiology, Vol.7(1) 2017: 7-9</p> 2017-01-31T00:00:00+00:00 ##submission.copyrightStatement## Detection of microbial proliferation & assessment of antibacterial activity of spices collected from various locations of Dhaka city, Bangladesh 2019-02-03T06:30:09+00:00 Md Aftab Uddin Khondakar Wahid Hasan Jubaida Binta Jamal <p>The current study was aimed to detect the presence of contaminating microorganisms in some commonly available spices collected from different areas of Dhaka city, Bangladesh and also to assess their antibacterial activity. Out of eight samples under this study, total viable bacterial count (TVBC) was observed within the range of 10<sup>6 </sup>to 10<sup>7</sup>cfu/g. Among the specific bacterial isolates <em>Staphylococcus </em>spp.<em>, Pseudomonas </em>spp. and <em>Escherichia coli </em>were found in almost all the samples within the margin of 10<sup>2</sup> to 10<sup>6</sup> cfu/g. Alarmingly <em>Salmonella </em>spp. and <em>Vibrio </em>spp. were presumptively identified in one and two samples respectively. Besides every sample showed the presence of fungus with load up to 10<sup>3</sup> cfu/g. Antibacterial activity of these samples was tested against seven laboratory isolates. <em>Syzygium aromaticum </em>showed highest activity against as many as five bacterial isolates while <em>Cinnamomum verum </em>was active against four isolates. On the other contrary, both <em>Zingiber officinale </em>and <em>Laurus nobilis </em>demonstrated their antibacterial activity only against <em>Staphylococcus </em>spp. and <em>Klebsiella </em>spp. respectively.</p> <p>Stamford Journal of Microbiology, Vol.7(1) 2017: 10-13</p> 2017-01-31T00:00:00+00:00 ##submission.copyrightStatement## Influence of tannery wastes and the surrounding environment of tannery industries on microbial growth and proliferation 2019-02-03T06:30:11+00:00 Nayan Chandra Das Farzana Hossaini Saurab Kishore Munshi <p>The present study was carried out to assess the degree of microbiological proliferation in tannery wastes and the surrounding environment of the tannery industries. In this regard, a total of 8 tannery waste (n=4) and environmental (n=4) samples were tested. All the samples contained a huge load of bacteria and fungi in an average of 10<sup>8</sup> cfu/g or ml. An extended numbers of pathogenic bacteria were recovered. Among the pathogenic bacteria, <em>Staphylococcus </em>spp. was predominant. Most of the samples exhibited the presence of <em>Pseudomonas </em>spp. <em>Salmonella </em>spp. and fecal coliform were found each in one sample. <em>Bacillus </em>spp., <em>Escherichia coli</em>, <em>Klebsiella </em>spp. and <em>Vibrio </em>spp. were found in few samples. The average load of the pathogens was 10<sup>4</sup> cfu/g or ml. All the pathogenic isolates were found to be multidrug resistant. Higher resistance was found against penicillin and streptomycin. Tannery waste after lather treatment sample showed antibacterial activity against all the pathogens tested. Overall, presence of pathogenic microorganisms with multidrug resistance traits may pose serious public health threats.</p> <p>Stamford Journal of Microbiology, Vol.7(1) 2017: 14-18</p> 2017-01-31T00:00:00+00:00 ##submission.copyrightStatement## Microbiological quality analysis of domestic water collected from the slum area’s people in Dhaka city 2019-02-03T06:30:15+00:00 Ifra Tun Nur Rakhi Baishnab Niger Sultana Tethee <p>Over the last few consecutive decades along with urbanization slum population has been raised. But unfortunately unsatisfactory water supply and sanitation systems are the main obstacles of their livelihood and they are suffering from many diseases. So our present study attempted to focus on the existence of pathogenic microorganisms in domestic water used by slum population along with drug resistant pattern of the isolated bacteria. Total 20 samples were collected and processed for microbiological action. Significant numbers of coliform bacteria especially <em>Escherichia coli </em>and <em>Klebsiella </em>spp. were found within the range of 10<sup>2</sup> to 10<sup>4</sup> cfu/ml. Conversely almost all samples exhibited fecal contamination except sample 5 and 6. Growth of fungus and other pathogenic bacteria such as <em>Staphylococcus </em>spp. and <em>Vibrio </em>spp<em>. </em>also found in almost every sample. Total 10 available drugs were used against the identified isolates<em>. </em>Most of the identified bacteria were found to be sensitive against commonly used antibiotics. Resistance was found only against ampicilin, amoxycilin and vancomycin.</p> <p>Stamford Journal of Microbiology, Vol.7(1) 2017: 19-22</p> 2017-01-31T00:00:00+00:00 ##submission.copyrightStatement## Study on the microbiological potential of biofertilizer applied on Brassica oleracea (cauliflower) 2019-02-03T06:30:17+00:00 Mrityunjoy Acharjee <p>Plant nutrients are vital component of sustainable agriculture as they are essential for the production of crops and healthy food. Chemical fertilizer or pesticides may have huge influence to increase the heavy metal and resistant microbes in soil as well as in crops. In order to provide an experimental evidence on the positive impact of bio-fertilizer instead of chemical fertilizer on agricultural field, present study attempted to collect 50 samples of <em>Brassica oleracea </em>(25 were treated with bio-fertilizer and 25 were chemically treated) from different agricultural land of rural area in Bangladesh. The samples were processed to examine the microbiological and clinical aspects of both bio-fertilizer and chemical fertilizer on vegetables through several common, traditional and replicable cultural and biochemical tests. Both samples were found to be contaminated with total viable bacteria and fungi up to the range 10<sup>8</sup> &amp; 10<sup>6</sup>cfu/g, respectively. The elevated range of pathogenic contamination (<em>Staphylococcus </em>spp., <em>Bacillus </em>spp., <em>Pseudomonas </em>spp.) was found in both samples within the range of 10<sup>2</sup> to 10<sup>6</sup>cfu/g. In case of biofertilizer treated vegetable the contamination of <em>Staphylococcus </em>spp. was prominent up to 10<sup>6 </sup>cfu/g and the same existence was found for chemically treated vegetable. <em>Bacillus </em>spp. and <em>Pseudomonas </em>spp. were found 10<sup>4</sup> &amp; 10<sup>5</sup> cfu/g, respectively in biofertilizer treated vegetable while the contamination was noticed up to 10<sup>2</sup> &amp; 10<sup>4</sup> cfu/g in chemically treated vegetable respectively. Another important era of this study is drug resistant pattern, most of the isolates exhibited resistance against commonly used antibiotics while several isolates were noted to be multi-drug resistant (MDR). The drug resistance strains were remarkably high in chemically treated vegetable whereas maximum antibiotics were extremely effective against the bacteria isolated from biofertlizer treated vegetable</p> <p>Stamford Journal of Microbiology, Vol.7(1) 2017: 23-27</p> 2017-01-31T00:00:00+00:00 ##submission.copyrightStatement## Complete microbiological analysis of citrus fruits and the effect of heat on microbial load & antimicrobial activity 2019-02-03T06:30:19+00:00 Sheikh Al Mamun Farahnaaz Feroz <p>Citrus fruits are very popular both for raw consumption as well as juices, jam and jelly. Besides the nutritional properties, citrus fruits exhibit some antimicrobial properties by containing polymethoxylated flavones, flavonoids, steroids, saponins, alkaloids, reducing sugars, terpenoids etc. But sometimes such fruits can be contaminated with bacteria which find their ways in the consumers causing different disease conditions. The current study revealed the microbial load of Lemon, Lotkone, Orange, Malta and Amoloki and the study showed complete absence of <em>Klebsiella </em>spp. and <em>Escherichia coli</em>. The highest total viable bacterial and fungal count was 4.2<em>×</em>10<sup>4</sup> cfu/g and 2.0<em>×</em>10<sup>5</sup> cfu/g respectively. <em>Pseudomonas </em>spp. was the highest predominating bacteria with lower degree of contamination by <em>Listeria </em>spp. and <em>Staphylococcus </em>spp. Applying heat at 60<sup>o</sup>C for 30 minutes, 1 hour and 2 hours proved the reduction of bacteria over time. Antibacterial activity after heat treatment for all cases (after 30 minutes, 1 hour and 2 hours). Antibacterial activity was lowest after 2 hours of heat treatment for amoloki and there was no such activity at all for Amra after 2 hours. Other citrus fruits surprisingly showed no antibacterial activity after heat treatment.</p> <p>Stamford Journal of Microbiology, Vol.7(1) 2017: 28-32</p> 2017-01-31T00:00:00+00:00 ##submission.copyrightStatement## Detection of drug resistant bacteria in different ice creams available in local market of Dhaka city 2019-02-03T06:30:21+00:00 Md Musa Howlader Tahmina Shammi <p>Sound microbiological quality of ice cream should be ensured for being a widely popular dairy food in the world. Present study was conducted to determine the microbiological quality of different ice cream samples available in Dhaka, Bangladesh. Total 3 ice cream samples were collected and processed to detect the microbiological quality as well as drug resistant trait of the isolates through several conventional Kirby Bauer method. All the samples were found to be contaminated with the total viable bacteria and fungi within the range of 1.2×10<sup>4</sup> cfu/ml to 4.3×10<sup>6</sup> cfu/ml. The presence of <em>E. coli, Staphylococcus aureus </em>was also observed up to 10<sup>4</sup> cfu/ml. However, the fecal contamination was totally absent in all the samples. Antibiotic profile of two isolates was measured against 8 commonly used antibiotics and both <em>E.coli </em>and <em>Staphylococcus aureus </em>were found to be resistant against more than one antibiotics. Appropriate hygienic and storage condition should be maintained concerning the health safety of consumers.</p> <p>Stamford Journal of Microbiology, Vol.7(1) 2017: 33-35</p> 2017-01-31T00:00:00+00:00 ##submission.copyrightStatement##