TY - JOUR AU - Saha, B AU - Bal, M PY - 2016/07/30 Y2 - 2024/03/28 TI - Emergence of Multi-Drug Resistant Clinical Strains of Staphylococcus aureus JF - International Journal of Natural Sciences JA - Int J Nat Sci VL - 3 IS - 1 SE - Articles DO - 10.3329/ijns.v3i1.28579 UR - https://banglajol.info/index.php/iJNS/article/view/28579 SP - 1-6 AB - <p>Indiscriminate and wide spread use of antibiotics has lead to the development of multi-drug-resistant strains of pathogenic <em>Staphylococcus aureus. </em>Information regarding increase (in percentage) of existing resistance as well as emergence of new resistance to different antibiotics used for staphylococcal infections are insufficient. This study explores a comparative analysis of growing resistance to different antibiotics mainly ampicillin, methicillin, erythromycin, gentamicin, clindamycin and vancomycin against <em>S. aureus </em>isolated from Kolkata hospitals during two phases. During first phase (126) and second phase (67) non-repeat clinical strains of <em>S. aureus </em>obtained from different hospitals of Kolkata were identified by standard biochemical methods. However, PCR amplification of <em>nuc </em>gene and rDNA was also performed for identification of <em>S. aureus</em>. Antibiotic susceptibility pattern was determined by Disc Agar Diffusion tests and <em>mecA </em>was identified by PCR. Comparative analysis of antibiotic resistance pattern of the strains isolated during two phases showed significant difference (p=0.05) with 75% increase of resistance to erythromycin followed by 30% increase to ampicillin, chloramphenicol and streptomycin with the appearance of vancomycin resistance. Gentamicin and methicillin resistance have increased by 22% and 7% respectively. On the other hand, <em>mecA </em>was obtained by PCR from vancomycin resistant <em>S. aureus </em>strain, which was also resistant to methicillin, erythromycin and clindamycin. This study reveals tremendous increase of resistance to erythromycin and a remarkable increase to other antibiotics with emergence of multidrug- resistant clinical strains of <em>S. aureus</em>. This trend in increasing resistance to the commonly used antibiotics against <em>S. aureus </em>cannot be controlled until and unless antibiotics are used more prudently.</p><p>International Journal of Natural Sciences (2013), 3(1-4) 1-6</p> ER -