Cycloartenyl Ferulate downregulates lipopolysaccharide stimulated iNOS mRNA via NF-kB suppression in RAW 264.7 macrophages

Authors

  • Md Shafiqul Islam Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo
  • Hideki Ushio Laboratory of Marine Biochemistry, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo
  • Masatoshi Hori Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo
  • Hiroshi Ozaki Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo

DOI:

https://doi.org/10.3329/ajmbr.v2i4.30992

Keywords:

cycloartenyl ferulate, iNOS, COX-2, NF-kB, RAW 264.7 macrophage

Abstract

Cycloartenyl ferulate (CAF) is a major bioactive phytosteryl ferulate purified from rice bran ?- oryzanol. Previously we reported that CAF ameliorates DSS-induced colitis in mice. The present study was undertaken to investigate the effects of CAF on LPS (lipopolysaccharide) stimulated murine RAW 264.7 macrophages. Immunohistochemistry analysis demonstrated that LPS (10ng/mL) treatment exhibited nuclear translocation of NF-?B-p65 in RAW macrophages, which was markedly inhibited CAF (30?M). LPS (10ng/mL) stimulation for 1-4 hours significantly upregulated iNOS and COX-2 mRNA in RAW 264.7 macrophages, but COX-2 mRNA was faster than that of iNOS mRNA. Macrophages pretreated with CAF greatly inhibited the LPS stimulated iNOS mRNA in a dose dependent manner (1-30?M), but CAF weakly inhibited COX-2 mRNA. Interestingly, CAY 10404 (COX-2 inhibitor) inhibited LPS stimulated iNOS mRNA, but not COX-2. In addition, PGE2 (1?M) upregulated iNOS mRNA but did not show any remarkable effects on NF-?B-p65 nuclear translocation in RAW macrophages. PD98059 (p44/42 MAP kinase inhibitor) inhibited iNOS mRNA, but not COX-2. On the other hand, PD169316 (p38 MAP kinase inhibitor) neither inhibited iNOS mRNA nor COX-2. Our results suggest that iNOS mRNA expression by LPS is mediated via p44/42 MAP kinase pathway in RAW 264.7 macrophages, which depends on the preceding expression of COX-2 expression. CAF downregulates iNOS mRNA via an inhibition of nuclear translocation of NF-?B with different mode of action on COX-2 gene expression.

Asian J. Med. Biol. Res. December 2016, 2(4): 523-531

Downloads

Download data is not yet available.
Abstract
865
PDF
621

Author Biography

Md Shafiqul Islam, Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo



Downloads

Published

2017-01-23

How to Cite

Islam, M. S., Ushio, H., Hori, M., & Ozaki, H. (2017). Cycloartenyl Ferulate downregulates lipopolysaccharide stimulated iNOS mRNA via NF-kB suppression in RAW 264.7 macrophages. Asian Journal of Medical and Biological Research, 2(4), 523–531. https://doi.org/10.3329/ajmbr.v2i4.30992

Issue

Section

Articles