Epidemiological investigation and phylogenetic analyses of contagious ecthyma virus from goat in Bangladesh

Authors

  • Md Thoufic Anam Azad Bangladesh Livestock Research Institute, Savar, Dhaka and Department of Animal Husbandry and Veterinary Science, Rajshahi University, Rajshahi
  • Sukumar Saha Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh
  • Md Shahin Alam Bangladesh Livestock Research Institute, Savar, Dhaka
  • Papia Monoura Bangladesh Livestock Research Institute, Savar, Dhaka
  • Md Giasuddin Bangladesh Livestock Research Institute, Savar, Dhaka
  • Shake Mohammed Shariful Islam Upazila Livestock Officer, Meherpur Sadar, Meherpur
  • Mahbub Jang Fatey Ali Taimur Bangladesh Livestock Research Institute, Savar, Dhaka
  • Jahangir Alam Bangladesh Livestock Research Institute, Savar, Dhaka and National Institute of Biotechnology, Ganakbari, Savar, Dhaka-1349

DOI:

https://doi.org/10.3329/ajmbr.v2i4.30996

Keywords:

contagious ecthyma, epidemiologic characteristics, molecular characterization and phylogenetic analysis

Abstract

The aim of this study was to know the epidemiologic characteristics of contagious ecthyma and molecular characterization of the contagious ecthyma virus or ORF virus (ORFV). Sixty four households having 293 goats in Chuadanga district of Bangladesh were surveyed and 30 scab materials were collected from ORF affected goat. Virus detection by PCR was attempted. Sequencing was done for characterization. Goats (n=70) in 15 out of 64 (23.44%) households were found to be infected with ORF. The overall morbidity, mortality and case fatality was 23.89 (70/293), 1.02 (3/293) and 4.29% (3/70), respectively. A proliferative lesion in mouth, lips and gum was found in 100.00% affected goats. Besides, proliferative lesions were also found in legs (19.00%), body (13.00%), and ears (10.00%) of goats. Highest morbidity (29.00%) was found in goats of 7 months-1years aged group and lowest (2.05%) in goats of 2-3 years or more. About 79.00% goats were affected during January-March, 2011 and 21.00% during OctoberDecember, 2010. About 93.33% (28/30) scab samples were found positive by PCR. For molecular characterization PCR amplified viral interlukin-10 (vIL10) gene of 5 different ORFV were sequenced. One virus obtained from sheep and four from goat. Goat isolates were found very closely related (97.20-98.60%) to each other while sheep virus has 95.20-97.20% homology with goat viruses and clustered separately. Our isolates were closely related to Norwegian sheep isolates. It is concluded that ORFV is circulating in Bangladesh with high morbidity but low mortality and all age group of goats are susceptible to this disease. Circulating viruses are closely related to each other.

Asian J. Med. Biol. Res. December 2016, 2(4): 555-561

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Author Biography

Md Thoufic Anam Azad, Bangladesh Livestock Research Institute, Savar, Dhaka and Department of Animal Husbandry and Veterinary Science, Rajshahi University, Rajshahi



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Published

2017-01-23

How to Cite

Azad, M. T. A., Saha, S., Alam, M. S., Monoura, P., Giasuddin, M., Islam, S. M. S., Taimur, M. J. F. A., & Alam, J. (2017). Epidemiological investigation and phylogenetic analyses of contagious ecthyma virus from goat in Bangladesh. Asian Journal of Medical and Biological Research, 2(4), 555–561. https://doi.org/10.3329/ajmbr.v2i4.30996

Issue

Vol. 2 No. 4 (2016)

Section

Articles