Interleukin-33, an interleukin-1 like cytokine accelerates cell migration in IEC-6 cells

Authors

  • Md Shafiqul Islam Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202
  • Masatoshi Hori Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo
  • Hiroshi Ozaki Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo

DOI:

https://doi.org/10.3329/ajmbr.v2i4.30999

Keywords:

IEC-6 intestinal epithelial cell, interleukin-33, migration, wound healing

Abstract

Interlukin-33 (IL-33) is a novel cytokine of the IL-1 superfamily that is expressed mainly in stromal cells, such as epithelial and endothelial cells. It is believed to be involved in inflammation and carcinogenesis. Recently we have reported that IL-33 is up regulated in azoxymethane-dextran sulfate sodium salt (AOM/DSS)- induced in colitis in mice. In this study we have investigated the biological activity of IL-33 in cells migration in IEC-6 epithelial cells. Our wound healing assay demonstrated that IL-33 significantly enhanced cell migration towards the wound edges compared with the untreated control cells. Trans-well migration assay in presence of cells culture and wound stimulation in the lower chamber, IL-33 exhibited significant chemoattractant activity for the cells migration. On the other hand, in absence of cells culture and wound stimulation in the lower chamber, IL-33 neither produced remarkable chemokine, nor chemoattractant activity for the cells migration. These findings indicate that IL-33 interacts with wound-stimulated soluble cellular product(s) in the lower chamber to accelerate cell migration.

Asian J. Med. Biol. Res. December 2016, 2(4): 577-581

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Author Biography

Md Shafiqul Islam, Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202



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Published

2017-01-23

How to Cite

Islam, M. S., Hori, M., & Ozaki, H. (2017). Interleukin-33, an interleukin-1 like cytokine accelerates cell migration in IEC-6 cells. Asian Journal of Medical and Biological Research, 2(4), 577–581. https://doi.org/10.3329/ajmbr.v2i4.30999

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