Isolation, identification and antibiogram profiles of enterovirulent Escherichia coli from diarrhoeic goat in some selected areas of Rangpur district of Bangladesh

Authors

  • Md Rashed Kamal Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200
  • Md Fakhruzzaman Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200
  • Mir Rowshan Akter Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200
  • Md Atiqul Haque Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200

DOI:

https://doi.org/10.3329/ajmbr.v4i1.36819

Keywords:

Escherichia coli, diarrhoea, goat, antibiogram

Abstract

Enterovirulent Escherichia coli remain as an important etiological agent of goat diarrhoea in Bangladesh. The present study was designed with a view to isolate and identifies E. coli from field cases. For this purpose, a total of 135 faecal samples (85 from diarrhoeic and 50 from apparently healthy goat) were collected during the period from January 2012 to July 2012 from different areas in Rangpur District. It was found that the prevalence of E. coli was higher (18.82 %) in diarrhoeic goats while it was lower (14.00 %) in non diarrhoeic goats. Age wise distribution of E. coli isolates were 26.42% in day old to 1 year, 10.53% in 1-2 years and 11.36% in above 2 years age of goat respectively. All the isolates of E. coli revealed greenish black colony with metallic sheen in Eosine methylene blue agar, bright pink color smooth transparent colony in MacConkey agar and slight pinkish smooth colony in Salmonella-Shigella agar. Gram stain and hanging drop techniques were performed with the cultured bacteria. Biochemical properties of the isolates were studied, and antibiotic sensitivity test was done by agar disk diffusion method. In Gram stain, the organisms revealed Gram negative, small rod shaped, occurs singly or paired. Biochemically, all of the isolates showed fermentation of dextrose, sucrose and maltose with the production of acid and gas, negative result to Voges-Proskauer test, positive result to Methylred test and differential result to Indol test. All the isolates of E. coli were highly sensitive to ciprofloxacin and gentamicin while moderately sensitive to colistin, livofloxacin and azithromycin and less sensitive to ceftraexon and tetracyclin and resistant to amoxycillin, ampicillin, erythromycin, and neomycin. Therefore, ciprofloxacin and gentamicin may be the antibiotics of first choice, and colistin, livofloxacin and azithromycin may be the second choice among the test antibiotics for the treatment of illness caused by these bacteria.

Asian J. Med. Biol. Res. March 2018, 4(1): 36-43

Downloads

Download data is not yet available.
Abstract
958
PDF
897

Author Biography

Md Rashed Kamal, Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200



Downloads

Published

2018-06-07

How to Cite

Kamal, M. R., Fakhruzzaman, M., Akter, M. R., & Haque, M. A. (2018). Isolation, identification and antibiogram profiles of enterovirulent Escherichia coli from diarrhoeic goat in some selected areas of Rangpur district of Bangladesh. Asian Journal of Medical and Biological Research, 4(1), 36–43. https://doi.org/10.3329/ajmbr.v4i1.36819

Issue

Vol. 4 No. 1 (2018)

Section

Articles

License

Authors who publish with this journal agree to the following terms / The author(s) affirm(s) that:

  1. The manuscript submitted is based on authors own research and is original work.
  2. Authors certify that we all participated in the research work and preparation of the manuscript in a substantive way.
  3. Authors also declare that they have read and approved the manuscript.
  4. Authors further declare that the manuscript has not been published in part or full and is not being submitted or considered for publication in part or full elsewhere.
  5. Any material included in the manuscript does not violate copyright or other rights of anyone. 
  6. Authors  also affirm that the article contains no vilifying or unlawful statements and does not contain material or instructions that might cause harm or injury to the Editor-in-Chief/Editors of the Journal and the public.
  7. Authors assure Asian J. Med. Biol. Res. and the Editor-in-Chief/Editors of the Journals, and hold them harmless from any loss, expense or damage occurred by a claim or suit by a third party for copyright violation, or any suit arising out of any violation of the foregoing warranties as a result of publication of my/our article. 
  8. In consideration of authors manuscript submitted, authors hereby grant Asian J. Med. Biol. Res. unlimited, worldwide, permanent royalty-free, right to publish, use, dispense, license, transmit, display, exhibit, record, store, translate, digitize, broadcast, reproduce and archive, in any format or medium, whether now known or developed hereafter.

All materials related to manuscripts, accepted or rejected, including photographs, original figures etc., will be kept by Asian J. Med. Biol. Res. for one year following the editor’s decision. These materials will then be destroyed.