Antibiotic sensitivity and resistant pattern of bacteria isolated from table eggs of commercial layers considering food safety issue
DOI:
https://doi.org/10.3329/ajmbr.v4i4.40103Keywords:
antibiotic; sensitivity; resistance; table eggs; commercial layers; food safetyAbstract
The present study was carried out during the period of June 2016 to June 2017 to evaluate the antibiotic sensitivity and resistant pattern of bacteria isolated from table eggs of commercial layers considering food safety issue. A total of 200 egg samples (100 for egg shell surface and 100 for egg content) were collected from different retail markets of Dhaka city in sterile polythene bags in a view to prevent extraneous contamination and transported to the laboratory immediate after collection using icebox. The samples were inoculated onto nutrient broth and nutrient agar plates aerobically at 37°Cfor isolation. The isolated organisms were identified based on staining, motility, colony morphology and biochemical tests. The isolated bacteria were also subjected to characterize their antibiotic sensitivity. About 74% egg samples (148 out of 200 samples) were positive for microbial contamination. Among them 100 (100 %) samples had their shells contaminated with microbes of different genera; however, only 48 (48%) growths were observed from the egg contents. The major contaminants are Escherichia coli (34.64%), Coagulase positive Staphylococcus (24.29%), Salmonella spp. (20.71%) followed by Coagulase negative Staphylococcus (10%), Pseudomonas spp. (6.43%) and Bacillus spp. (3.93%). The isolated bacteria E. coli, Coagulase positive Staphylococcus, Salmonella spp. and Pseudomonas spp. showed their greatest sensitivity against ciprofloxacin, ceftriaxone and azithromycin whereas resistant against tetracycline, amoxicillin and ampicillin. There is potential for these antibiotic-resistant bacteria to be transferred to humans through contaminated eggs and are of public health concern from food safety point of view.
Asian J. Med. Biol. Res. December 2018, 4(4): 323-329
Downloads
460
384
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms / The author(s) affirm(s) that:
- The manuscript submitted is based on authors own research and is original work.
- Authors certify that we all participated in the research work and preparation of the manuscript in a substantive way.
- Authors also declare that they have read and approved the manuscript.
- Authors further declare that the manuscript has not been published in part or full and is not being submitted or considered for publication in part or full elsewhere.
- Any material included in the manuscript does not violate copyright or other rights of anyone.
- Authors also affirm that the article contains no vilifying or unlawful statements and does not contain material or instructions that might cause harm or injury to the Editor-in-Chief/Editors of the Journal and the public.
- Authors assure Asian J. Med. Biol. Res. and the Editor-in-Chief/Editors of the Journals, and hold them harmless from any loss, expense or damage occurred by a claim or suit by a third party for copyright violation, or any suit arising out of any violation of the foregoing warranties as a result of publication of my/our article.
- In consideration of authors manuscript submitted, authors hereby grant Asian J. Med. Biol. Res. unlimited, worldwide, permanent royalty-free, right to publish, use, dispense, license, transmit, display, exhibit, record, store, translate, digitize, broadcast, reproduce and archive, in any format or medium, whether now known or developed hereafter.
All materials related to manuscripts, accepted or rejected, including photographs, original figures etc., will be kept by Asian J. Med. Biol. Res. for one year following the editors decision. These materials will then be destroyed.
