Measurement of circulating insulin-like peptide 3 and testosterone concentrations in pre-pubertal, tropical male goats
DOI:
https://doi.org/10.3329/ajmbr.v4i4.40111Keywords:
enzyme immunoassay; INSL3; male goats; pre-pubertal; serum; testosteroneAbstract
The present study attempted to: (1) simplify the existing enzyme immunoassay (EIA) for the measurement of insulin-like peptide 3 (INSL3) concentrations in goats (2) to measure circulating INSL3 and testosterone in pre-pubertal Jamnapari X local crossbred goats (3) to examine the relationships among INSL3 concentration, testosterone concentration, scrotal circumference and body weight during the pre-pubertal age. Serial blood samples were collected from normal pre-pubertal male Jamnapari X local crossbred goats (n = 6) at the ages of 19 to 28 weeks. Serum INSL3 was measured using a recently reported EIA with modifications to the original procedure. The detection ranges of the INSL3 and testosterone assays were 0.08 to 80 ng/mL and 0.01 to 40 ng/mL, respectively. The intra-assay coefficient of variations were 3.79% for INSL3 (n = 6) and 3.72% (n = 6) for testosterone. Serum INSL3 concentrations ranged from 13.62 ± 3.25 to 22.45 ± 6.09 ng/mL (mean ± SEM) in pre-pubertal goats. Those concentrations increased (p < 0.05) from 20 (13.62 ± 3.25 ng/mL) to 22 (22.45 ± 6.09 ng/mL) weeks of age. Testosterone concentrations ranged from 0.30 ± 0.07 to 1.22 ± 0.43 ng/mL in pre-pubertal goats. A significant drop was observed in testosterone concentrations at 23 weeks of age. INSL3 was correlated (r = 0.58; p < 0.05) with scrotal circumference while no significant correlation was observed among other tested parameters. A rapid, sensitive EIA system was simplified to quantify INSL3 in goats, by simplifying the existing procedure. Different serum INSL3 and testosterone dynamics were found from 19 to 28 weeks of age of the goats. Compared with testosterone, INSL3 dynamics seemed to be more consistent with the age of pre-pubertal goats and showed a relationship with the testicular growth.
Asian J. Med. Biol. Res. December 2018, 4(4): 383-388
Downloads
372
300
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms / The author(s) affirm(s) that:
- The manuscript submitted is based on authors own research and is original work.
- Authors certify that we all participated in the research work and preparation of the manuscript in a substantive way.
- Authors also declare that they have read and approved the manuscript.
- Authors further declare that the manuscript has not been published in part or full and is not being submitted or considered for publication in part or full elsewhere.
- Any material included in the manuscript does not violate copyright or other rights of anyone.
- Authors also affirm that the article contains no vilifying or unlawful statements and does not contain material or instructions that might cause harm or injury to the Editor-in-Chief/Editors of the Journal and the public.
- Authors assure Asian J. Med. Biol. Res. and the Editor-in-Chief/Editors of the Journals, and hold them harmless from any loss, expense or damage occurred by a claim or suit by a third party for copyright violation, or any suit arising out of any violation of the foregoing warranties as a result of publication of my/our article.
- In consideration of authors manuscript submitted, authors hereby grant Asian J. Med. Biol. Res. unlimited, worldwide, permanent royalty-free, right to publish, use, dispense, license, transmit, display, exhibit, record, store, translate, digitize, broadcast, reproduce and archive, in any format or medium, whether now known or developed hereafter.
All materials related to manuscripts, accepted or rejected, including photographs, original figures etc., will be kept by Asian J. Med. Biol. Res. for one year following the editors decision. These materials will then be destroyed.
