Isolation, identification and pathogenicity of fungal organisms causing postharvest rot of sweet oranges, cucumber and lettuce in Sharada Market, Kano State-Nigeria
DOI:
https://doi.org/10.3329/ajmbr.v5i4.45266Keywords:
mycological studies; sweet oranges; cucumber; lettuce; isolation; identificationAbstract
Isolation, identification and pathogenicity of fungal organisms responsible for the postharvest spoilage of sweet oranges, cucumber and lettuce at Sharada market in Kano metropolis, Nigeria was carried out in the present study. Samples showing symptoms of rot were collected from the retailers in the market and conveyed to the laboratory using polyethene bags. Small portion from rotten parts were cut and surface sterilized in 1% of Sodium hydrochloride and rinsed in several changes of sterile distilled water. They were plated on Potato Dextrose Agar (PDA) and observed for fungal growth. Identification was done macroscopically and microscopically. For pathogenicity test, healthy sweet oranges, cucumber and lettuce were plugged with pure cultures of the fungal isolates, thus, frequency of occurrence and disease severity of the pathogen were evaluated. Seven fungi namely Rhizopus spp., Aspergillus flavus, Mucor spp., Aspergillus fumigatus, Aspergillus niger, Fusarium oxysporum, Rhizoctonia spp. and Fusarium oxysporum were isolated. Severity of decay ranged from 6.39–46.15% for all fungal isolates, while the controls showed 0%. Pathogenic microorganisms on fruits and vegetables are a potential health hazard to man and animals following ingestion. Proper handling from the farm as well as during storage and the avoidance of mixing of diseased ones with the healthy ones were identified as important factors in preventing loss and health complication at the event of consumption. The use of suitable chemical treatment of the orange is also recommended as means of reducing economic loss due to fungal pathogens.
Asian J. Med. Biol. Res. June 2019, 5(4): 286-291
Downloads
355
299
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms / The author(s) affirm(s) that:
- The manuscript submitted is based on authors own research and is original work.
- Authors certify that we all participated in the research work and preparation of the manuscript in a substantive way.
- Authors also declare that they have read and approved the manuscript.
- Authors further declare that the manuscript has not been published in part or full and is not being submitted or considered for publication in part or full elsewhere.
- Any material included in the manuscript does not violate copyright or other rights of anyone.
- Authors also affirm that the article contains no vilifying or unlawful statements and does not contain material or instructions that might cause harm or injury to the Editor-in-Chief/Editors of the Journal and the public.
- Authors assure Asian J. Med. Biol. Res. and the Editor-in-Chief/Editors of the Journals, and hold them harmless from any loss, expense or damage occurred by a claim or suit by a third party for copyright violation, or any suit arising out of any violation of the foregoing warranties as a result of publication of my/our article.
- In consideration of authors manuscript submitted, authors hereby grant Asian J. Med. Biol. Res. unlimited, worldwide, permanent royalty-free, right to publish, use, dispense, license, transmit, display, exhibit, record, store, translate, digitize, broadcast, reproduce and archive, in any format or medium, whether now known or developed hereafter.
All materials related to manuscripts, accepted or rejected, including photographs, original figures etc., will be kept by Asian J. Med. Biol. Res. for one year following the editors decision. These materials will then be destroyed.
