Antagonistic effect of Lactobacillus spp. on experimentally Vibrio spp. infected Penaeus monodon
DOI:
https://doi.org/10.3329/ajmbr.v6i2.48077Keywords:
Lactobacillus spp.; Vibrio spp.; shrimp; In-vitro; In-vivoAbstract
The research was aimed to determine the present status of probiotics (Lactobacillus spp.) and pathogenic bacteria (Vibrio spp.) of culture pond as well as to determine whether the isolated probiotic Lactobacillus spp. can act as a controlling agent on experimentally pathogenic Vibrio spp. infected Penaeus monodon. In In-vitro test of gills and intestinal tracts, the potential antagonistic activity of Lactobacillus spp. toward Vibrio spp. was gradually observed at 4th hour of probiotic treatment. At 12th hour the viable count of Vibrio spp. was drastically reduced in gill from 2.33×105 to 1.03×103 (CFUg-1) and in intestinal tract 2.35×105 to 6.43×102 (CFUg-1). While, in In-vivo test, in case of muscle, gills and intestinal tract antagonistic activity of Lactobacillus spp. toward Vibrio spp. was noticed after 9 hours, 21 hours and 27 hours respectably of probiotic injected shrimps. However, only the result of in-vitro challenge test revealed that, Lactobacillus spp. significantly reduced the Vibrio spp. viable count of the gills of the selected samples (P=0.037). The investigation showed antagonistic effect of probiotic (Lactobacillus spp.) on experimentally Vibrio spp. infected shrimp.
Asian J. Med. Biol. Res. June 2020, 6(2): 311-315
Downloads
254
248
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms / The author(s) affirm(s) that:
- The manuscript submitted is based on authors own research and is original work.
- Authors certify that we all participated in the research work and preparation of the manuscript in a substantive way.
- Authors also declare that they have read and approved the manuscript.
- Authors further declare that the manuscript has not been published in part or full and is not being submitted or considered for publication in part or full elsewhere.
- Any material included in the manuscript does not violate copyright or other rights of anyone.
- Authors also affirm that the article contains no vilifying or unlawful statements and does not contain material or instructions that might cause harm or injury to the Editor-in-Chief/Editors of the Journal and the public.
- Authors assure Asian J. Med. Biol. Res. and the Editor-in-Chief/Editors of the Journals, and hold them harmless from any loss, expense or damage occurred by a claim or suit by a third party for copyright violation, or any suit arising out of any violation of the foregoing warranties as a result of publication of my/our article.
- In consideration of authors manuscript submitted, authors hereby grant Asian J. Med. Biol. Res. unlimited, worldwide, permanent royalty-free, right to publish, use, dispense, license, transmit, display, exhibit, record, store, translate, digitize, broadcast, reproduce and archive, in any format or medium, whether now known or developed hereafter.
All materials related to manuscripts, accepted or rejected, including photographs, original figures etc., will be kept by Asian J. Med. Biol. Res. for one year following the editors decision. These materials will then be destroyed.