Genetic diversity analysis of advanced rice lines for salt tolerance using SSR markers

Authors

  • Tanoy Singha Department of Genetics and Plant Breeding, Bangladesh Agricultural University (BAU), Mymensingh-2202, Bangladesh
  • Md Asif Mahamud Department of Agricultural Chemistry, Khulna Agricultural University (KAU), Khulna-9100, Bangladesh
  • Shahin Imran Department of Agronomy, Khulna Agricultural University (KAU), Khulna-9100, Bangladesh
  • Newton Chandra Paul Department of Agronomy, Khulna Agricultural University (KAU), Khulna-9100, Bangladesh
  • Md Najmol Hoque Department of Biochemistry and Molecular Biology, Khulna Agricultural University (KAU), Khulna-9100, Bangladesh
  • Tusher Chakrobarty Rice Farming Systems (RFS) division, Bangladesh Rice Research Institute (BRRI), Gazipur-1701, Bangladesh
  • Md Asadulla Al Galib Agronomy division, Bangladesh Rice Research Institute (BRRI), Gazipur-1701, Bangladesh
  • Lutful Hassan Department of Genetics and Plant Breeding, Bangladesh Agricultural University (BAU), Mymensingh-2202, Bangladesh

DOI:

https://doi.org/10.3329/ajmbr.v7i2.55001

Keywords:

diversity; marker; PIC; salt tolerance; SSR; UPGMA

Abstract

Twenty-two rice lines were used to evaluate salt tolerance at the Laboratory of Biotechnology Division of Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh. Four SSR markers viz. AP3206f, RM1287, RM7075, and RM10793 were used to screen the germplasm for salt tolerance. SSR analysis revealed that the number of alleles per locus ranged from 3 to 5 with an average of 4.25 alleles per locus. Polymorphism Information Content (PIC) values ranged from 0.4762 (RM7075) to 0.7524 (AP3206f) with an average of 0.61 per locus. The highest genetic diversity (0.7810) was observed in loci AP3206f, and the lowest genetic diversity (0.5620) was observed in loci RM7075 with a mean diversity of 0.6663. The genotypes with genetic similarity clustered together in the dendrogram based on UPGMA method and we observed seven major clusters where cluster I contained most of the genotypes. Cluster I, II, III, IV, V, VI, and VII contained 6, 1, 2, 4, 4, 4 and 1 genotypes, respectively. These results revealed that marker AP3206f would be best in screening 22 rice genotypes followed by RM1287, RM7075, and RM10793 according to PIC values. These findings can have the potential role for further improvement of salinity tolerance rice genotypes through marker-assisted breeding.

Asian J. Med. Biol. Res. 2021, 7 (2), 214-221

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Published

2021-06-30

How to Cite

Singha, T. ., Mahamud, M. A. ., Imran, S. ., Paul, N. C. ., Hoque, M. N., Chakrobarty, T. ., Al Galib, M. A. ., & Hassan, L. . (2021). Genetic diversity analysis of advanced rice lines for salt tolerance using SSR markers. Asian Journal of Medical and Biological Research, 7(2), 214–221. https://doi.org/10.3329/ajmbr.v7i2.55001

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Section

Research Articles