Evaluation of an antimalarial herbal mixture and each extract for DNA and chromosomal mutations in Swiss albino mice and Allium cepa cells

Authors

  • Akinboro Akeem Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
  • Rufai Muhamed Akinlabi Zoology Department, Osun State University, Osogbo, Osun State, Nigeria
  • Ogunbameru Aanuoluwapo Dorcas Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
  • Oladosu Bolaji Esther Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
  • Taiwo Modinat Opeyemi Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria

DOI:

https://doi.org/10.3329/ajmbr.v7i3.56135

Keywords:

DNA fragmentation; Azadirachta indica; Alstonia boonei; chromosomes aberrations

Abstract

Toxicological evaluation of herbal medicines is necessary because of possible adverse effects that may be associated with their consumption. This study screened antimalarial herbal recipe (containing leaves of Azadirachta indica and stem-bark of Alstonia boonei) and its individual plant’s extract for DNA and chromosomes mutation potentials following the DNA fragmentation and Allium cepa assays. Superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) activity of the recipe and each extract was determined. The kinds of phytochemicals present in them were determined using the FTIR technique. Water extracts of A. indica, and A.boonei at all the tested doses caused significantly lower DNA fragmentations than those of the controls. However, at 25.0% and 50.0% recipe, there was no significant difference in the percentage fragmented DNA compared to the positive control (0.05% sodium azide). Cell division was significantly inhibited by the extracts and recipe, chromosomal aberrations were not dose dependently induced and were significantly lowered than that caused by sodium azide (positive control). The individual extracts and their recipe significantly inhibited Root growth. However, 12.5% recipe promoted root growth that was not significantly different from that of distilled water (negative control). SOD and CAT activities of each of the extracts and their recipe were dose dependent and significantly higher than those of the controls. Water extract of A. indica significantly suppressed generation of malondialdehyde compared to water extract of A. boonei and recipe as well as the control. The individual extracts and their recipe contained phenolic phytochemicals. The obtained results show that extract of A. indica, A. boonei and their recipe have good antioxidant properties with strong mitodepressive and root growth inhibitory effects except at 12.5% recipe. However, A. indica extract seems to have least cyto-muta-genotoxic effects than water extract of A. boonei and the recipe in mice and A. cepa cells.

Asian J. Med. Biol. Res. 2021, 7 (3), 249-259

Downloads

Download data is not yet available.
Abstract
7
PDF
9

Downloads

Published

2021-09-30

How to Cite

Akeem, A. ., Akinlabi, R. M. ., Dorcas, O. A. ., Esther, O. B. ., & Opeyemi, T. M. . (2021). Evaluation of an antimalarial herbal mixture and each extract for DNA and chromosomal mutations in Swiss albino mice and Allium cepa cells. Asian Journal of Medical and Biological Research, 7(3), 249–259. https://doi.org/10.3329/ajmbr.v7i3.56135

Issue

Vol. 7 No. 3 (2021)

Section

Research Articles

License

Copyright (c) 2021 Akinboro Akeem, Rufai Muhamed Akinlabi, Ogunbameru Aanuoluwapo Dorcas, Oladosu Bolaji Esther and Taiwo Modinat Opeyemi

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms / The author(s) affirm(s) that:

  1. The manuscript submitted is based on authors own research and is original work.
  2. Authors certify that we all participated in the research work and preparation of the manuscript in a substantive way.
  3. Authors also declare that they have read and approved the manuscript.
  4. Authors further declare that the manuscript has not been published in part or full and is not being submitted or considered for publication in part or full elsewhere.
  5. Any material included in the manuscript does not violate copyright or other rights of anyone. 
  6. Authors  also affirm that the article contains no vilifying or unlawful statements and does not contain material or instructions that might cause harm or injury to the Editor-in-Chief/Editors of the Journal and the public.
  7. Authors assure Asian J. Med. Biol. Res. and the Editor-in-Chief/Editors of the Journals, and hold them harmless from any loss, expense or damage occurred by a claim or suit by a third party for copyright violation, or any suit arising out of any violation of the foregoing warranties as a result of publication of my/our article. 
  8. In consideration of authors manuscript submitted, authors hereby grant Asian J. Med. Biol. Res. unlimited, worldwide, permanent royalty-free, right to publish, use, dispense, license, transmit, display, exhibit, record, store, translate, digitize, broadcast, reproduce and archive, in any format or medium, whether now known or developed hereafter.

All materials related to manuscripts, accepted or rejected, including photographs, original figures etc., will be kept by Asian J. Med. Biol. Res. for one year following the editor’s decision. These materials will then be destroyed.