Dietary phosphorus and feeding regimens modulate egg production, quality, and serum parameters in laying hens aged 61-80 weeks
DOI:
https://doi.org/10.3329/ajmbr.v11i4.75619Keywords:
feed restriction, nutrient utilization, Lohmann brown hens, performance traits, blood biochemistryAbstract
Effective management of nutrients and feeding practices is essential to sustain egg production, maintain egg quality, and support the health of aging laying hens while optimizing resource use. This study examined the influence of dietary available phosphorus (AP) and quantitative feeding levels (QFL) on egg production, egg quality, and serum biochemical traits in Lohman Brown laying hens. A total of 540 hens, 60 weeks old, were assigned in a 2×3 factorial design, combining two AP levels (0.32% and 0.45%) with three feeding levels (90%, 95%, and 100% of standard intake). Each treatment included five replicates of 18 hens, and the experiment lasted 20 weeks following a 7-day acclimation period. Results showed that production performance was significantly affected by feeding level (P < 0.01), whereas AP levels had no significant impact. Egg production declined as feed restriction increased (P < 0.01), though no significant difference occurred between the 95% and 100% feeding groups. Egg weight, daily egg mass, and feed conversion ratio also decreased linearly with higher restriction (P < 0.01). Most egg quality traits were unaffected, except yolk color, which was greater at 0.45% AP (P < 0.01). Serum albumin decreased in the 90% feeding group (P < 0.05) but remained similar between 95% and 100% feeding levels. No significant interactions were detected between AP and QFL for serum biochemical indices. Overall, feeding hens at 95% of the recommended intake with 0.32% AP effectively maintained egg production, quality, and health, indicating that moderate feed restriction combined with adequate phosphorus can reduce feed costs without compromising performance in late-phase laying hens.
Asian J. Med. Biol. Res. 2025, 11(4), 88-96
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References
Abdurofi I, MM Ismail, HAW Kamal and BH Gabdo, 2017. Economic analysis of broiler production: A case study of integrated poultry farms in Peninsular Malaysia. Int. Food Res. J., 24: 761-766.
Anene DO, Y Akter, PC Thomson, P Groves and CJ Oshea, 2023. Effect of restricted feeding on hen performance, egg quality and organ characteristics of individual laying hens. Anim. Nutr., 14: 141-151.
Baradaran N, MH Shahir, HR Taheri and MR Bedford, 2021. Effect of sequential feeding of phosphorus-deficient diets and high-dose phytase on efficient phosphorus utilization in broiler chickens. Livest. Sci., 243: 104368.
Bello A and DR Korver, 2019. Long-term effects of Buttiauxella sp. phytase on performance, eggshell quality, apparent ileal Ca and P digestibility, and bone properties of white egg layers. Poult. Sci., 98: 4848-4859.
Cheng X and Z Ning, 2023. Research progress on bird eggshell quality defects: a review. Poult. Sci., 102: 102283.
Dijkslag MA, RP Kwakkel, E Martin-Chaves, C Alfonso-Carrillo and A Navarro-Villa, 2023. Long-term effects of dietary calcium and phosphorus level, and feed form during rearing on egg production, eggshell quality, and bone traits in brown laying hens from 30 to 89 wk of age. Poult. Sci., 102: 102618.
Ding Y, X Bu, N Zhang, L Li and X Zou, 2016. Effects of metabolizable energy and crude protein levels on laying performance, egg quality and serum biochemical indices of Fengda-1 layers. Anim. Nutr., 2: 93-98.
Eltahan HM, S Cho, MM Rana, AA Saleh, MAM Wadaan, M Alagawany IH Kim and HM Eltahan, 2023. Dietary exogenous phytase improve egg quality, reproductive hormones, and prolongs the lifetime of the aging Hy-Line brown laying hens fed nonphytate phosphorus. Poult. Sci., 102: 102895.
Eltahan HM, S Cho, MM Rana, AA Saleh, AE Elkomy, MAM Wadaan, M Alagawany, IH Kim and HM Eltahan, 2023. Dietary exogenous phytase improve egg quality, reproductive hormones, and prolongs the lifetime of the aging Hy-Line brown laying hens fed nonphytate phosphorus. Poult. Sci., 102: 102895.
Fathi MM, A Galal, UM Ali and OK Abou-Emera, 2019. Physical and mechanical properties of eggshell as affected by chicken breed and flock age. British Poult. Sci., 60: 506-512.
Gu H, Z Yan, B Zhang, X Chen, A Geng, Y Zhang, J Cao, J Zhang, L Zeng, Z Wang, H Liu and Q Chu, 2025. Impact of eggshell color diversity on hatchability, translucency, and quality traits in Beijing-you chicken eggs. Animals, 15: 2595.
Ibigbami DJ, AA Adeyemo, OJ Odutayo, KR Idowu, MO Sogunle and OMO Idowu, 2024. Effects of feed form and quantitative feed restriction on blood profile of broiler chickens. Nigerian J. Anim. Prod., 51: 1-14.
Jing M, S Zhao, A Rogiewicz, BA Slominski and JD House, 2018. Assessment of the minimal available phospho rus needs of laying hens: Implications for phosphorus management strategies. Poult. Sci., 97: 2400-2410.
Lee MH, EJ Cho, ES Choi, MH Bang and SH Sohn, 2016. The effect of hen age on egg quality in commercial layer. Korean J. Poult. Sci., 43: 253-261.
Li P, R Wang, H Jiao, X Wang, J Zhao and H Lin, 2018. Effects of dietary phosphorus level on the expression of calcium and phosphorus transporters in laying hens. Front. Physiol., 9: 627.
Li X, D Zhang, TY Yang and WL Bryden, 2016. Phosphorus bioavailability: A key aspect for conserving this critical animal feed resource with reference to broiler nutrition. Agriculture, 6: 25.
Liu Y, VA Uyanga, H Jiao, X Wang, J Zhao, Y Zhou and H Lin, 2023. Effects of feeding strategies on eggshell quality of laying hens during late laying period. Poult. Sci., 102: 102406.
Mellouk N, C Rame, M Marchand, C Staub, JL Touze, E Venturi, M Merchand, A Travel, P Chartrin, F Lecompte, L Ma, P Froment and J Dupont, 2018. Effect of different levels of feed restriction and fish oil fatty acid supplementation on fat deposition by using different techniques, plasma levels and mRNA expression of several adipokines in broiler breeder hens. PLoS One, 13: e0191121.
Moreira RF, ER Freitas, FS Sucupira, ALF Diogenes, MS Abe and FWS Araujo, 2012. Effect of feed restriction with voluntary hay intake on the performance and quality of laying hen eggs. Act. Sci. Anim. Sci., 34: 149-154.
Nie W, Y Yang, J Yuan, Z Wang and Y Guo, 2013. Effect of dietary nonphytate phosphorus on laying performance and small intestinal epithelial phosphate transporter expression in Dwarf pink-shell laying hens. J. Anim. Sci. Biotechnol., 4: 34.
Nóbrega IPTD, MP Reis, FAH Morillo, LFVBD Freitas, LC Bittencourt, JBK Fernandes and NK Sakomura, 2022. Dynamics of growth and egg traits in three dietary balanced protein scenarios applied for laying hens. Animals, 12: 1371.
Okongwu GC, UN Okonkwo and MU Mbachu, 2025. Effective of quantitative feed restriction on the performance of laying hens. J. Occup. Train., 9: 127-137.
Olawumi SO, 2014. Effect of short-term feed restriction on production traits of brown and black plumage commercial layer strains at late phase of egg production. American J. Agric. For., 2: 33-38.
Olawuni KA, CO Ubosi and SO Alaku, 2012. Effects of feed restriction on egg production and egg quality of exotic chickens during their second year of production in Sudano-Sahelian environment. Anim. Feed Sci. Technol., 38: 1-9.
Osman AMA, MA Toson, SA Abdel-Latif, HHM Hassanien, TMA Marwan, 2010. Effects of fasting on reproductive performance of laying hens. Egypt. Poult. Sci., 30: 1031-1057.
Panda AK, S Sahoo and A Kumar, 2024. Effect of variation in nutrient density on production performance and serum biochemical parameters of Rhode Island Red laying hens. Indian J. Poult. Sci., 59: 11-14.
Park J, YJ Heo, DH Kim YB Kim, BY Kwon, JY Song and KW Lee, 2024. Nutritional and physiological responses to dietary phosphorus levels and phytase in pullets and laying hens. Poult. Sci., 103: 103886.
Pongmanee K, I Kiihn and DR Korver, 2020. Effects of phytase supplementation on eggshell and bone quality, and phosphorus and calcium digestibility in laying hens from 25 to 37 wk of age. Poult. Sci., 99: 2595-2607.
Rana MM, CI Lim, MSK Sarker, AA Bhuiyan, and KS Ryu, 2023. Effect of dietary phosphorus and restricted feeding on performance, egg quality and serum biochemical traits of laying hens at the second production phase. Bang. J. Agric., 47: 119–132.
Rao SVR, KN Kumari, SS Paul, P Bhukya, MVLN Raju, PSP Kumar and TS Krishna, 2025. Optimization of dietary energy intake to minimize egg production during market crises in White Leghorn laying hens. J. Appl. Poult. Res., 34: 100517.
Rodehutscord M, V Sommerfeld, CR Angel and DR Korver, 2023. Minimum phosphorus requirements for laying hen feed formulation. Poult. Sci., 102: 102344.
Sekeroglu A, YE Senturk, B Tainika, M Duman and A Akyol, 2024. The impact of laying hen age, egg-laying time, cage tier, and cage direction on egg quality traits in hens in an enriched cage system. Braz. Poult. Sci., 26: eRBCA-2024-1902.
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Copyright (c) 2025 Md Masud Rana, Md Sazedul Karim Sarker, Md Yousuf Ali, Md Razibul Hassan, Md Aftabuzzaman
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