Indiscriminate use of ciprofloxacin antibiotic in broiler reveals high antibiotic residues in broiler meat
DOI:
https://doi.org/10.3329/ajmbr.v11i2.78548Keywords:
antimicrobial resistance, withdrawal period, hematological analysis, residue detection, food safetyAbstract
In Bangladesh, excessive and haphazard antibiotic use is frequently observed in the poultry industry, which is a global issue in terms of antibiotic residue and resistance. This study aimed to detect ciprofloxacin residues in edible tissues and assess their impact on broilers' hematological and major enzymatic parameters. Thirty-day-old Cobb-500 broiler chicks were used to examine the effects of controlled and uncontrolled ciprofloxacin usage during the period from June to December 2021 at Bangladesh Agricultural University (BAU). On the 14th day, the chicks were randomly assigned to control, discriminate, and indiscriminate antibiotic groups. Treatment commenced from day 14, with the discriminate group receiving ciprofloxacin at 100 mg/L in drinking water for 7 days followed by a 7-day withdrawal period. Conversely, the indiscriminate group received ciprofloxacin at the same concentration for 14 days without any withdrawal period. Daily body weights of the broiler were recorded. On day 14, the body weights across all groups showed no significant differences, but by day 28, the indiscriminate group exhibited a significantly higher mean body weight (1601.43±15.50 g). Thin layer chromatography (TLC) analysis revealed the presence of ciprofloxacin residues in all samples from the indiscriminate group, while no residues were detected in the control group. In the discriminate group, residues were found in 2 liver samples, 3 kidney samples, 1 spleen sample, and 1 breast muscle sample, but not in thigh muscle samples. Hematological analysis indicated significant differences in total erythrocyte count (TEC), hemoglobin (Hb), and erythrocyte sedimentation rate (ESR) among the groups, with no significant differences in packed cell volume (PCV). Enzymatic analysis of blood serum for ALT and AST showed no substantial changes between treated and control birds. Therefore, the controlled use of ciprofloxacin in the poultry sector is crucial to safeguard human health.
Asian J. Med. Biol. Res. 2025, 11(2), 37-44
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References
Al-Mashhadany DA, A Nahla, AM Zaki and VS Mohammad, 2018. Detection of antibiotic residues among poultry meat in Erbil city and impact of thermal processing on remnants. Res. J. Life Sci. Bioinform. Pharma. Chem. Sci., 3: 237-247.
Billah MM, SMM Rana, MS Hossain, AKM Saifuddin, SKMA Islam, Z Naim and S Barua, 2014. Determination of the presence and pharmacokinetic profile of ciprofloxacin by TLC and HPLC method respectively in broiler chicken after single oral administration. J. Antibiot., 67: 745-748.
Castanon JIR, 2007. History of the use of antibiotic as growth promoters in European poultry feeds. Poult. Sci., 86: 2466-2471.
Choma IM, 2003. TLC separation of fluoroquinolones: searching for better selectivity. J. Liq. Chromatogr. Relat. Technol., 26: 2673-2685.
Cieśla Ł, M Waksmundzka-Hajnos and J Sherma, 2019. Thin layer chromatography. In: Ewing's Analytical Instrumentation Handbook. Edited by: Grinberg N and S Rodriguez, CRC Press, pp. 921-942.
Costa MC, JA Bessegatto, AA Alfieri, JS Weese, JA Filho and A Oba, 2017. Different antibiotic growth promoters induce specific changes in the cecal microbiota membership of broiler chicken. PLoS One, 12: e0171642.
Csikó G, 2023. Residues relating to the veterinary therapeutic or growth-promoting use and abuse of medicines. In: Present Knowledge in Food Safety. Edited by: Knowles ME, LE Anelich, AR Boobis and B Popping, Elsevier, pp. 96–113.
Dahiya J, D Wilkie, A Van Kessel and M Drew, 2006. Potential strategies for controlling necrotic enteritis in broiler chickens in post-antibiotic era. Anim. Feed Sci. Technol., 129: 60-88.
Demir E, Ş Sarica, M Özcan and M Sui Mez, 2003. The use of natural feed additives as alternatives for an antibiotic growth promoter in broiler diets. Br. Poult. Sci., 44: 44-45.
Fletouris DJ, 2000. Drug residues in foods: Pharmacology: Food safety, and analysis. CRC Press, USA.
Haag G, GH Marin and J Errecalde, 2016. Quantification of residual ciprofloxacin in feathers of broiler by high performance liquid chromatography-fluorescence after oral drug administration. J. Adv. Pharm. Technol. Res., 7: 2-5.
Islam KS, SSU Mahmuda and MHB Kabir, 2016. Antibiotic usage patterns in selected broiler farms of Bangladesh and their public health implications. J. Public Health Dev. Ctries., 2: 276-284.
Islam M, B Belal, S Dash, M Islam and M Hasan, 2024a. Chronic exposure of ciprofloxacin antibiotic residue above the MRL level and its pathophysiological effects in mice. Curr. Res. Compliment. Altern. Med., 8: 249.
Islam MS, MS Islam and MR Hasan, 2024b. Precision and validation of thin layer chromatography analytical technique to analyze and monitoring antibiotic residues in poultry products and byproducts. J. Pharmacol. Clin. Res., 10: 555786.
Islam MS, MZ Islam and MS Islam, 2019. Discriminate and indiscriminate use of amoxicillin and its effects on hematological parameters of broiler. Asian J. Med. Biol. Res., 5: 153-157.
Islam MS, S Sachi, S Dash and MS Islam, 2023. Detection and mitigation of antibiotic residues in poultry products and byproducts. Asian Australas. J. Food Saf. Secur., 7: 33-39.
Kasprzak M, T Hetmański and E Kulczykowska, 2006. Changes in hematological parameters in free-living pigeons (Columba livia f. urbana) during the breeding cycle. J. Ornithol., 147: 599-604.
Metli M, Y Yakar Y and Y Tekeli, 2015. Determination of antibiotic residues in chicken liver by liquid chromatography-tandem mass spectrometry. Adıyaman Univ. J. Sci., 5: 120-131.
Mitchell JM, MW Griffiths M, SA McEwen, WB McNab and AJ Yee, 1998. Antimicrobial drug residues in milk and meat: causes, concerns, prevalence, regulations, tests, and test performance. J. Food Prot., 61: 742-756.
Miyakawa MEF, NA Casanova and MH Kogut, 2024. How did antibiotic growth promoters increase growth and feed efficiency in poultry? Poult. Sci., 103: 103278.
Popelka P, J Nagy, R Germuska, S Marcinčák, P Jevinova and A De Rijk, 2005. Comparison of various assays used for detection of beta-lactam antibiotics in poultry meat. Food Addit. Contam., 22: 557-562.
Sarker YA, MM Hasan, TK Paul, SZ Rashid, MN Alam and MH Sikder, 2018. Screening of antibiotic residues in chicken meat in Bangladesh by thin layer chromatography. J. Adv. Vet. Anim. Res., 5: 140-145.
Sattar S, MM Hassan, SKMA Islam, M Alam, SA Faruk, S Chowdhury and AKM Saifuddin, 2014. Antibiotic residues in broiler and layer meat in Chittagong district of Bangladesh. Vet. World, 7: 738-743.
Senanayake SSHMML, JGS Ranasinghe, R Waduge, K Nizanantha and PABD Alexander, 2015. Changes in the serum enzyme levels and liver lesions of broiler birds reared under different management conditions. Trop. Agric. Res., 26: 584-595.
Sherma J and B Fried, 2003. Handbook of thin-layer chromatography. CRC press, USA.
Van Boeckel TP, C Brower, M Gilbert, BT Grenfell, SA Levin, TP Robinson, A Teillant and R Laxminarayan, 2015. Global trends in antimicrobial use in food animals. Proc. Natl. Acad. Sci. USA, 112: 5649-5654.
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Copyright (c) 2025 Md Rakibul Hasan, Md Shakil Islam, Md Mominul Islam, Jahagir Alam, Al Wasef, Md Tanvir Hasan, Kazi Rafiq, Md Shafiqul Islam
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