Development of blast-resistant rice varieties through marker-assisted selection: Development of blast-resistant rice varieties

M K A Nadim; M M Islam; M I Hoque; M J Hasan; M I Uddin

doi:10.3329/bjagri.v49i1.74041

Authors

M K A Nadim Biotechnology Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh
M M Islam Biotechnology Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh;
M I Hoque Entomology Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh
M J Hasan Horticulture Division, BINA, Mymensingh. Bangladesh
M I Uddin Biotechnology Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh

DOI:

https://doi.org/10.3329/bjagri.v49i1.74041

Keywords:

Backcrossing, Marker-Assisted Selection, Pyricularia oryzae, SSR Marker

Abstract

The blast of rice caused by Pyricularia oryzae fungus plays a vital role in reducing the yield of rice (Oryza sativa L.) in Bangladesh. Rice blast is one of the major constraints on rice productivity in many countries, including Bangladesh. An experiment was conducted from November 2022 to April 2023 at the Biotechnology Division of the Bangladesh Institute of Nuclear Agriculture aimed to developing blast-resistant rice varieties using marker-assisted selection. Three rice varieties were used: BRRI dhan48, BRRI dhan58, and IRBL9-W. SSR markers were employed for F₁ confirmation, selecting 14 plants for backcrossing. PCR analysis and gel electrophoresis confirmed F₁ plants through polymorphic markers. A total of 97 BC₁F₁ seeds were produced. The study contributes to developing blast-resistant rice lines, crucial for mitigating yield losses caused by Pyricularia oryzae, addressing a significant threat to rice production in Bangladesh.

Bangladesh J. Agri. 2024, 49(1): 41-51

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Development of blast-resistant rice varieties through marker-assisted selection