NPR1 gene transformation as assessed by germ cell in situ transformation pathway into Siraitia grosvenorii

Authors

  • Jing-Jian Li Guangxi Institute of Botany, The Chinese Academy of Sciences, Guilin 541006
  • Xin Liu Guangxi Institute of Botany, The Chinese Academy of Sciences, Guilin 541006
  • Bo Zhao Guangxi Institute of Botany, The Chinese Academy of Sciences, Guilin 541006
  • Wen-Lan Li College of Life Science, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004

DOI:

https://doi.org/10.3329/bjb.v44i2.38513

Keywords:

Siraitia grosvenorii, NPR1 gene, Germ cell, In situ transformation, Transgenosis

Abstract

NPR1 gene was transformed into Siraitia grosvenorii (Swingle) C. Jeffrey ex. A.M. Lu and Zhi Y. Zhang by germ cell in situ transformation. Ovary injection, cutting chapiter, and chapiter spreading treatments were applied in this study. The transgenic plants were selected using hygromycin screening and confirmed by PCR testing, genome integrated with NPR1 gene in transgenic plants was analyzed by Southern hybridization. Results showed that three treatments could produce transgenic plants. Some of the transgenic plants were selected for tobacco mosaic virus inoculation testing, which showed a higher level of resistance to tobacco mosaic virus than non-transgenic controls.

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Published

2018-10-13

How to Cite

Li, J.-J., Liu, X., Zhao, B., & Li, W.-L. (2018). NPR1 gene transformation as assessed by germ cell in situ transformation pathway into Siraitia grosvenorii. Bangladesh Journal of Botany, 44(2), 245–250. https://doi.org/10.3329/bjb.v44i2.38513

Issue

Vol. 44 No. 2 (2015)

Section

Articles

License

© Bangladesh Botanical Society

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