Calcium alginate entrapment of Aspergillus nidulans IMPP-0785 laccase for enhanced enzyme catalytic ability, thermost ability and dye-decolorization

Authors

  • Malik Muhammad Yousaf Arid Zone Research Institute (AZRI), Bahawalpur, Pakistan
  • Muhammad Majeed University of Gujrat, Gujrat, Pakistan
  • Mumtaz Hussain Arid Zone Research Institute (AZRI), Bahawalpur, Pakistan
  • Muhammad Jahangir Shah Arid Zone Research Institute (AZRI), Bahawalpur, Pakistan
  • Bashir Ahmad Arid Zone Research Institute (AZRI), Bahawalpur, Pakistan
  • Hafiz Muhammad Zia Ullah Ghazali Oilseed Research Station Khanpur, Pakistan
  • Rao Wali Muhammad PARC research and training Station, BZU Multan, Pakistan

DOI:

https://doi.org/10.3329/bjb.v47i4.47380

Keywords:

Laccase, Aspergillus nidulans IMPP-0785, Optimal conditions

Abstract

Production of laccase using a solid-state culture of Aspergillus nidulans IMPP-0785 was optimized using barley bran. Aspergillus nidulans IMPP-0785 laccase optimum activity was observed with filtrate (29.15 U/g) as compared to supernatant and unfiltered. Free laccase and supernatant gave best results at 30.24 and 25.95 U/g, respectively for 20 min incubation time. The entrapped spores of A. nidulans IMPP-0785 showed maximal activity (16.12 U/g) at incubation time for 60 min. Immobilized laccase resulted optimal activity (29.24 U/g) for 25 min of incubation. Enzyme showed higher thermo stability of 34.28 U/g when incubated for 35 min.

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Author Biographies

Muhammad Majeed, University of Gujrat, Gujrat, Pakistan

Department of Botany

Hafiz Muhammad Zia Ullah Ghazali, Oilseed Research Station Khanpur, Pakistan

Assistant Plant Pathologist

Rao Wali Muhammad, PARC research and training Station, BZU Multan, Pakistan

Senior Scientific Officer

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Published

2018-12-31

How to Cite

Yousaf, M. M., Majeed, M., Hussain, M., Shah, M. J., Ahmad, B., Ghazali, H. M. Z. U., & Muhammad, R. W. (2018). Calcium alginate entrapment of Aspergillus nidulans IMPP-0785 laccase for enhanced enzyme catalytic ability, thermost ability and dye-decolorization. Bangladesh Journal of Botany, 47(4), 903–909. https://doi.org/10.3329/bjb.v47i4.47380

Issue

Vol. 47 No. 4 (2018)

Section

Articles

License

© Bangladesh Botanical Society

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