Genomic DNA isolation from scrophularieae dried leaves using a simple, high-throughput protocol

Authors

  • Mehrshid Riahi Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
  • Melina Babaei Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
  • Farrokh Ghahremaninejad Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

DOI:

https://doi.org/10.3329/bjb.v48i4.49083

Keywords:

CTAB, DNA extraction, Scrophularia, Verbascum

Abstract

This communication described efficient DNA extraction from Scrophularia and Verbascum samples. Modified Murray and Thompson modified Cota-Sànchez method and Bioflux kit methods were applied for the extraction of DNA. Among the different methods, Bioflux kit Plant DNA extraction kit, coupled with some modification was the best for extraction of high molecular weight DNA as long as the extracted DNA is quantified with fluorescence-based methods.

Downloads

Download data is not yet available.
Abstract
25
PDF
45

Downloads

Published

2019-12-31

How to Cite

Riahi, M., Babaei, M., & Ghahremaninejad, F. (2019). Genomic DNA isolation from scrophularieae dried leaves using a simple, high-throughput protocol. Bangladesh Journal of Botany, 48(4), 1231–1235. https://doi.org/10.3329/bjb.v48i4.49083

Issue

Vol. 48 No. 4 (2019)

Section

Short Communications

License

© Bangladesh Botanical Society

Authors are required to transfer the copyright of their articles to the journal. The Declaration form is available here http://www.bdbotsociety.org/journal/journal_pdf/declaration_form.pdf