Adventitious shoot regeneration from leaf and stem explants of Salix matsudana Koidz

Authors

  • Liu Xiaoxia State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
  • Sui Jinkai College of Agronomy and Biotechnology, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China
  • Zhang Jianguo State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
  • Luo Ying State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
  • Rao Guodong State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

DOI:

https://doi.org/10.3329/bjb.v49i2.49321

Keywords:

Adventitious shoot, Regeneration, Micropropagation, Salix matsudana

Abstract

Currently, the leaf and stem as explants for rapid propagation in vitro remained unknown in Salix matsudana. Multiple shoots were regenerated from leaf and stem (stem apex and stem with axil). The optimum medium for callus regeneration and shoots induction from leaf was on WPM medium containing 6.0 mg/l zeatin (Z) and 0.75 mg/l NAA. The optimum medium of shoot induction from stem apex was on MS medium supplemented with 8.0 mg/l Z and 1.0 mg/l NAA, and from stem with axil was on MS with 8.0 mg/l Z and 0.5 mg/l NAA. Rooting of regenerated shoots was obtained on the same medium supplemented with 1.0 mg/l activated charcoal.

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Published

2020-09-20

How to Cite

Xiaoxia, L., Jinkai, S., Jianguo, Z., Ying, L., & Guodong, R. (2020). Adventitious shoot regeneration from leaf and stem explants of Salix matsudana Koidz. Bangladesh Journal of Botany, 49(2), 395–400. https://doi.org/10.3329/bjb.v49i2.49321

Issue

Vol. 49 No. 2 (2020)

Section

Short Communications

License

© Bangladesh Botanical Society

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