Karyotype analysis with orcein and CMA from leaf base cells of <i>Rauvolfia serpentina</i> Benth. <i>et</i> Kurz

Authors

  • Syeda Sharmeen Sultana Department of Botany, University of Dhaka, Dhaka
  • ANM Rubaiyath Bin Rahman 1Dept. of Biotechnology and Genetic Engineering, Islamic University, Kushtia
  • Sheikh Shamimul Alam Department of Botany, University of Dhaka, Dhaka

DOI:

https://doi.org/10.3329/bjb.v38i1.5133

Keywords:

Karyotype, Rauvolfia serpentina, Fluorescent banding

Abstract

Many prominent darkly stained heterochromatic blocks were found in the interphase nuclei of Rauvolfia serpentina following orcein staining. The prophase chromosomes of this species became stained homogeneously throughout the entire length. This species was found to possess 2n = 20 metacentric chromosomes revealing symmetric karyotype but if the chromosome length (6.67 - 3.17 μm) is considered, it indicates asymmetric karyotype. Total GC-rich region was 15.62% of the total chromatin length. Eight CMA-positive bands on different locus revealed the accumulation of GC-rich repeats. The two entirely CMA-banded chromosomes were so unique that could be used as marker for this species.

Key words: Karyotype; Rauvolfia serpentina; Fluorescent banding

DOI: 10.3329/bjb.v38i1.5133

Bangladesh J. Bot. 38(1): 55-63, 2009 (June)

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How to Cite

Sultana, S. S., Rahman, A. R. B., & Alam, S. S. (2010). Karyotype analysis with orcein and CMA from leaf base cells of <i>Rauvolfia serpentina</i> Benth. <i>et</i> Kurz. Bangladesh Journal of Botany, 38(1), 111–113. https://doi.org/10.3329/bjb.v38i1.5133

Issue

Vol. 38 No. 1 (2009)

Section

Short Communications

License

© Bangladesh Botanical Society

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