Screening of homokaryotic protoclones of <i>Agaricus bisporus</i> (J. Lge) Imbach by colony characters and ISSR markers

Authors

  • Mahmudul Islam Nazrul Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan
  • Fan Xiao Lin Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan
  • Bian Yin-Bing Institute of Applied Mycology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan

DOI:

https://doi.org/10.3329/bjb.v39i1.5537

Keywords:

Agaricus bisporus (J. Lge) Imbach, Homokaryon, ISSR, Protoclone

Abstract

Among ten slow-growing protoclones of Agaricus bisporus (J. Lge) Imbach, all appressed colonies showed slower growth rate and spawn run, and inability to produce fruiting bodies in substrate. Seven of 40 inter-simple sequence repeat (ISSR) primers amplified 78 reproducible fragments, 48.93% were polymorphic, each producing 7 to 16 bands ranging from 0.10 to 2.10 kbp, sufficient to differentiate the protoclones from each other. Appressed protoclones were homoallelic at a number of loci that were heteroallelic in the parent, suggesting that they represented rare homokaryons. Thus, using morphological characters along with ISSR, polymorphisms could be useful for quick, easy, and accurate in distinguishing homo- and heterokaryotic isolates.

Key words: Agaricus bisporus (J. Lge) Imbach; Homokaryon; ISSR; Protoclone

DOI: 10.3329/bjb.v39i1.5537

Bangladesh J. Bot. 39(1): 119-122, 2010 (June)

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Published

2010-07-16

How to Cite

Nazrul, M. I., Lin, F. X., & Yin-Bing, B. (2010). Screening of homokaryotic protoclones of <i>Agaricus bisporus</i> (J. Lge) Imbach by colony characters and ISSR markers. Bangladesh Journal of Botany, 39(1), 119–122. https://doi.org/10.3329/bjb.v39i1.5537

Issue

Vol. 39 No. 1 (2010)

Section

Short Communications

License

© Bangladesh Botanical Society

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