Lateral bud induction and establishment of regeneration system of cassia mimosoides L.

Authors

  • Ya Li Yang School of Medicine, Jiaying University, Meizhou 514015, P.R. China.
  • Qian Qian Dai School of Medicine, Jiaying University, Meizhou 514015, P.R. China
  • Pei Ting He School of Medicine, Jiaying University, Meizhou 514015, P.R. China
  • Zhan Wen Lin School of Medicine, Jiaying University, Meizhou 514015, P.R. China
  • Sheng Yuan Zhang School of Medicine, Jiaying University, Meizhou 514015, P.R. China
  • Da Du Lin School of Medicine, Jiaying University, Meizhou 514015, P.R. China
  • Ying Liu College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, P.R. China
  • Ming Zhai School of Medicine, Jiaying University, Meizhou 514015, P.R. China.
  • Lu Bin Zhang Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas, Meizhou 514031, China

DOI:

https://doi.org/10.3329/bjb.v52i20.68214

Keywords:

Cassia mimosoides, Lateral bud induction, Regeneration system, Rooting

Abstract

To establish an efficient plant regeneration system of Cassia mimosoides, the 10-day-old seedlings were used as explants for inducing lateral buds. Results showed that the addition of BA and NAA for different ratios on the basis of 1/2 MS could effectively induce the production of lateral buds. By adding different additional elements, it was found that the growth of lateral buds was longer and stronger on the medium of 1/2 MS + 3.0 mg/l BA + 0.1 mg/l NAA + 4.0 mg/l Gln or 1/2 MS + 3.0 mg/l BA + 0.1 mg/l NAA + 1.0 mg/l AgNO3. Meanwhile, cutting with the lateral buds of tissue culture seedling, the rooting rate could reach 77.8% when 1/2 MS medium contained 0.2 mg/l NAA, although the root length was shorter. When the regenerated strong seedlings were transplanted into the soil, the survival rate was over 75%. Therefore, the best medium for lateral bud induction using 10-day-old seedlings as explants in C. mimosoides was 1/2 MS + 3.0 mg/l BA + 0.1 mg/l NAA. The growth of lateral buds can be promoted by the appropriate concentration of Gln and AgNO3, and the best rooting medium was 1/2 MS contained 0.2 mg/l NAA.

Bangladesh J. Bot. 52(2): 515-521, 2023 (June) Special

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Published

2023-08-31

How to Cite

Ya Li Yang, Qian Qian Dai, Pei Ting He, Lin, Z. W. ., Zhang, S. Y. ., Lin, D. D. ., Ying Liu, Zhai, M., & Zhang, L. B. . (2023). Lateral bud induction and establishment of regeneration system of cassia mimosoides L. Bangladesh Journal of Botany, 52(20), 515–521. https://doi.org/10.3329/bjb.v52i20.68214

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