Characterization of three citrullus lanatus (thunb.) Matsum. & nakai varieties by fluorescent chromosome staining and rapd analysis

Authors

  • Chandan Kumar Dash Cytogenetics Laboratory, Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh
  • Mahin Afroz Department of Botany, University of Barishal, Barishal-8254, Bangladesh.
  • Afsana Hossain Cytogenetics Laboratory, Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh
  • Syeda Sharmeen Sultana Cytogenetics Laboratory, Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh
  • Subroto Kumar Das Department of Botany, University of Barishal, Barishal-8254, Bangladesh.

DOI:

https://doi.org/10.3329/bjb.v52i4.70598

Keywords:

Karyotype, CMA, DAPI, RAPD, Citrullus lanatus

Abstract

Three commercial Citrullus lanatus varieties, namely Sonya, Kanya and Sweet black-2, were investigated with cyto-molecular techniques for characterization. Despite having similar somatic chromosome numbers and karyotype formulae (2n = 22), these three varieties had considerable diversities in Chromomycin A3 (CMA)- and 4′, 6-diamidino-2-phenylindole (DAPI)-banding patterns. The total somatic chromosomal length was longest in Sweet black-2 (71.44 ± 2.94 µm) and shortest in Sonya (42.31 ± 2.45 µm). Each variety's unique GC- and AT-rich chromatin composition was represented by a variety of numbers, percentages, and patterns of distribution. Eleven random primers were employed to evaluate the genetic diversity among three varieties. Each variety showed a distinct fingerprinting pattern with 88.72% polymorphism while having 10 of the same RAPD bands.

Bangladesh J. Bot. 52(4): 1033-1040, 2023 (December)

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Published

2023-12-31

How to Cite

Dash, C. K. ., Afroz, M., Hossain, A. ., Sultana, S. S., & Das, S. K. . (2023). Characterization of three citrullus lanatus (thunb.) Matsum. & nakai varieties by fluorescent chromosome staining and rapd analysis. Bangladesh Journal of Botany, 52(4), 1033–1040. https://doi.org/10.3329/bjb.v52i4.70598

Issue

Vol. 52 No. 4 (2023)

Section

Articles

License

© Bangladesh Botanical Society

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