Isolation and characterization of rhizobia and evaluation of their effect on radish (Raphanus Sativus ) growth

Authors

  • Gebremedhin Amare Weldemariam School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China
  • , Yang Yong Kui School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China
  • Etsay Mesele Ejigu School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China
  • Bruh Asmelash Gebremikael Department of Biotechnology, College of Dryland Agriculture and Natural Resources, Mekelle University, Mekelle, Tigray, Ethiopia

DOI:

https://doi.org/10.3329/bjb.v53i1.72284

Keywords:

Characterization, Isolation, Plant-Infection, Radish, Rhizobia

Abstract

This study focused on isolating and characterizing rhizobia from alfalfa root nodules and assessing their impact on Radish (Raphanus sativus L) growth. Fifteen isolates underwent morphological and biochemical analyses, revealing consistent traits with variations in colony elevation. Confirmatory tests identified all isolates as gram-negative, rod-shaped, catalase, and oxidase positive. Notably, 13 isolates exhibited citrate positivity, 14 urease positivity, and 2 gelatinase positivity. Ten isolates produced indole-3-acetic acid, nine solubilized phosphate, and all produced ammonia. R12 displayed the highest seed germination (100%). Significant effects (P < 0.05) on radish growth were observed, with R37 yielding the highest values for root length (19 cm), R5 for shoot length (9 cm), R5 for leaf number (12.6), R36 for fresh weight (8 g), and R37 for dry weight (5 g). These superior isolates hold potential as biofertilizers for enhancing radish growth.

Bangladesh J. Bot. 53(1): 185-192, 2024 (March)

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Published

2024-03-31

How to Cite

Weldemariam, G. A. ., Kui . Y. Y., Ejigu, E. M. ., & Gebremikael, B. A. . (2024). Isolation and characterization of rhizobia and evaluation of their effect on radish (Raphanus Sativus ) growth. Bangladesh Journal of Botany, 53(1), 185–192. https://doi.org/10.3329/bjb.v53i1.72284

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