Cloning, sequence analysis, and functional study of the PeDof-4 gene in response to drought stress

Authors

  • Ge Chen uangxi Academy of Agricultural Sciences/Guangxi Crop Genetic Improvement and Biotechnology Lab/ Key Laboratory of Passion fruit Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Nanning 530007, China.
  • Shun Song uangxi Academy of Agricultural Sciences/Guangxi Crop Genetic Improvement and Biotechnology Lab/ Key Laboratory of Passion fruit Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Nanning 530007, China.
  • Yongcai Huang Guangxi Academy of Agricultural Sciences/Guangxi Crop Genetic Improvement and Biotechnology Lab/ Key Laboratory of Passion fruit Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Nanning 530007, China.
  • Ping Jiang Guangxi Academy of Agricultural Sciences/Guangxi Crop Genetic Improvement and Biotechnology Lab/ Key Laboratory of Passion fruit Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Nanning 530007, China.
  • Jie Gui Guangxi Academy of Agricultural Sciences/Guangxi Crop Genetic Improvement and Biotechnology Lab/ Key Laboratory of Passion fruit Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Nanning 530007, China.
  • Xiongying Jiang Guilin Academy of Agricultural Sciences, Guilin, 541000, China
  • Chonglie Ma Guangxi Academy of Agricultural Sciences/Guangxi Crop Genetic Improvement and Biotechnology Lab/ Key Laboratory of Passion fruit Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Nanning 530007, China.
  • Haolan Mo Guilin Academy of Agricultural Sciences, Guilin, 541000, China
  • Bin Zhou Guilin Academy of Agricultural Sciences, Guilin, 541000, China
  • Dayong Fan Guilin Academy of Agricultural Sciences, Guilin, 541000, China
  • Liu Yang Guangxi Academy of Agricultural Sciences/Guangxi Crop Genetic Improvement and Biotechnology Lab/ Key Laboratory of Passion fruit Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Nanning 530007, China.

DOI:

https://doi.org/10.3329/bjb.v53i30.76605

Keywords:

Passion fruit, Dof, Sequence analysis, Cloning, Drought

Abstract

DNA-binding with one finger (Dof) was a functionally important gene in plant stress tolerance processes. The cloned full-length (849bp) passion fruit PeDof-4 gene was sequenced, which encodes  282 amino acids with the molecular formula C1274H1983N375O408S14, molecular weight 29529.87 u, and theoretical isoelectric point pI of 8.96. Evolutionary tree analysis showed that the PeDof-4-encoded proteins had a high degree of identity with Dof-encoded proteins in other plants. Blastp of the PeDof-4 encoded proteins revealed a closer affinity to the proteins encoded by the Dof of Theobroma cacao. The relative expression of the PeDof-4 gene was higher at 50% of the relative soil moisture content in the fluorescence qPCR experiments under different levels of drought stress.  Tissue-specific analysis showed that the highest expression of this gene was found in the roots. Overexpression of GUS gene under the PeDof-4 promoter inbidopsis thaliana showed that PeDof-4   could significantly respond to drought stress.

Bangladesh J. Bot. 52(3): 653-663, 2024 (September) Special

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Published

2024-10-14

How to Cite

Chen, G., Song, S., Huang, Y., Jiang, P., Gui, J., Jiang, X., Ma, C., Mo, H., Zhou, B., Fan, D., & Yang, L. (2024). Cloning, sequence analysis, and functional study of the PeDof-4 gene in response to drought stress. Bangladesh Journal of Botany, 53(30), 653–663. https://doi.org/10.3329/bjb.v53i30.76605

Issue

Vol. 53 No. 3 (Special) (2024)

Section

Articles

License

© Bangladesh Botanical Society

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