EMS dose optimization to develop large-scale mutants in rapeseed (Brassica L.)

Authors

  • Ummy Kulsum Oilseed Research Centre, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
  • Sheikh Hasna Habib Oilseed Research Centre, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
  • Pryanka Roy Oilseed Research Centre, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
  • Istiak Ahmed ASICT Division, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh.
  • Antara Samiha Oilseed Research Centre, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
  • F Begum Oilseed Research Centre, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
  • Md Abdul Latif Akanda Oilseed Research Centre, Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh

DOI:

https://doi.org/10.3329/bjb.v53i4.78129

Keywords:

Oilseed, Rapeseed, Mutation breeding, EMS dose, LD50, Survivability

Abstract

Mutation breeding is a great way to create variation in plant genomes, and Ethyl Methane Sulphonate (EMS) is commonly used for this purpose. To determine the optimum dose of EMS for developing large-scale mutants, B. napus and B. rapa (BARI Sarisha-18 and BARI Sarisha-20, respectively) were treated using six different concentrations (T0:0%, T1:0.1%, T2:0.2%, T3:0.3%, T4:0.4%, and T5:0.5%) of EMS. For B. napus, 100% germination was observed in T0, T1, and T2, while for B. rapa, it was in T0 and T1 treatment. The highest survival rates, 70% for B. napus and 60% for B. rapa, were recorded at T1 treatment. Morphological traits such as leaf number, shoot and root length, and fresh and dry weights was found highest in T1 treatment for both species. The LD50 was calculated at 0.16% for B. napus and 0.18% for B. rapa, indicating the ideal dose for generating a large number of mutants.

Bangladesh J. Bot. 53(4): 1077-1080, 2024 (December)

Downloads

Download data is not yet available.
Abstract
24
PDF
7

Downloads

Published

2024-12-30

How to Cite

Kulsum, U., Habib, S. H., Roy, P., Ahmed, I., Samiha, A., Begum , F., & Akanda, M. A. L. (2024). EMS dose optimization to develop large-scale mutants in rapeseed (Brassica L.). Bangladesh Journal of Botany, 53(4), 1077–1080. https://doi.org/10.3329/bjb.v53i4.78129

Issue

Vol. 53 No. 4 (2024)

Section

Short Communications

License

© Bangladesh Botanical Society

Authors are required to transfer the copyright of their articles to the journal. The Declaration form is available here http://www.bdbotsociety.org/journal/journal_pdf/declaration_form.pdf