Nitric Oxide Acts as an Early Diagnostic Tool for Intrauterine Diseases in Animal Health

Authors

  • Zinaida Artyushina Candidate of Veterinary Sciences, Assistant, Moscow State Academy of Veterinary Medicine and Biotechnology – MBA named after KI Scriabin, Moscow https://orcid.org/0000-0002-4519-8239
  • Sergey Fedotov Doctor of Veterinary Sciences, Professor, Russian State Agrarian University - Moscow Agricultural Academy named after KA Timiryazev, Moscow, Russia https://orcid.org/0000-0002-0004-3639
  • Vladimir Serezhenkov Candidate of Biological Sciences, Senior scientist employee of the laboratory of physical chemistry of biopolymers, Federal Research Center for Chemical Physics named after NN Semenov Russian Academy of Sciences, Moscow https://orcid.org/0000-0001-7620-3013
  • Nikita Sidnev Graduate Student, Moscow State Academy of Veterinary Medicine and Biotechnology – MBA named after KI Scriabin, Moscow https://orcid.org/0009-0002-7656-4059
  • Ilya Zherebtsov Graduate Student, Veterinarian, Assistant, Moscow State Academy of Veterinary Medicine and Biotechnology – MBA named after KI Scriabin, Moscow, Russia https://orcid.org/0009-0004-4062-2784
  • Reddy Gade Regan Graduate Student, Moscow State Academy of Veterinary Medicine and Biotechnology – MBA named after KI Scriabin, Moscow https://orcid.org/0009-0005-8764-0936
  • Zhora Muradyan Candidate of Biological Sciences, Assistant, Moscow State Academy of Veterinary Medicine and Biotechnology – MBA named after KI Scriabin, Moscow https://orcid.org/0000-0003-2516-7627

DOI:

https://doi.org/10.3329/bjid.v11i1.75566

Keywords:

Nitric oxide, intrauterine pathologies, diagnostics, biological models

Abstract

Background: Intrauterine pathologies in animals are a significant cause of reduced reproductive performance and symptomatic infertility, leading to economic losses in the livestock industry. Early diagnosis of these conditions is critical for effective treatment and management.

Objective: The study aimed to develop a method for the early diagnosis of intrauterine pathologies in animals by assessing nitric oxide (NO) levels using Electron Paramagnetic Resonance (EPR) spectroscopy.

Methodology: An experimental model was created using dairy Escherichia coli culture, which was injected into the uterus of pregnant rabbits under ultrasound guidance. Determination of the level of nitric oxide was accessed in the laboratories of the FSSI "Federal Research Center for Chemical Physics named after. N.N. Semenov" Russian Academy of Sciences. The study involved three groups of rabbits: two groups infected either intrauterine or intraperitoneally, and a control group. Group I (Intrauterine Infection) consisted of two rabbits. A suspension of Escherichia coli was injected directly into the uterus through the cervical canal to induce an intrauterine infection. Group II (Intraperitoneal Infection): This group included five rabbits. The Escherichia coli suspension was administered intraperitoneally, serving as a comparative model for systemic infection. Group III (Control): The remaining two rabbits served as the control group. These rabbits were not infected and were used to establish baseline measurements for comparison.

Results: The results demonstrated that the concentration of nitric oxide derivatives, specifically nitrites and N-nitrosamines (RNNO), significantly increased in the blood plasma of infected rabbits compared to the control group. These changes were observed during the early stages of inflammation, indicating the onset of intrauterine pathologies.

Conclusion: The developed method based on measuring nitric oxide derivatives in blood plasma is effective for the early diagnosis of intrauterine pathologies in animals.

Bangladesh Journal of Infectious Diseases, June 2024;11(1):52-58

 

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Published

2024-09-26

How to Cite

Artyushina, Z., Fedotov, S., Serezhenkov, V., Sidnev, N., Zherebtsov, I., Regan, R. G., & Muradyan, Z. (2024). Nitric Oxide Acts as an Early Diagnostic Tool for Intrauterine Diseases in Animal Health. Bangladesh Journal of Infectious Diseases, 11(1), 52–58. https://doi.org/10.3329/bjid.v11i1.75566

Issue

Vol. 11 No. 1 (2024)

Section

Original Articles

License

Copyright (c) 2024 Maxim Trushin

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