Degradation of Monochlorophenols by <i>Pseudomonas putida</i> CP1 in the Presence of Growth Supplements

Abstract

Influence of readily degradable additional sources of carbon and nitrogen on the degradation of monochlorophenols by Pseudomonas putida CP1 was investigated. The organism grew on all three isomers of monochlorophenols when supplied as the sole source of carbon and energy. Low concentrations (0.01 to 0.5%, w/v) of yeast extract enhanced degradation of monochlorophenols. The order in terms of rate of removal of monochlorophenols was 4-chlorophenol > 2-chlorophenol > 3-chlorophenol, both in the presence and absence of the growth supplements. The rate of removal of monochlorophenols was highest when carbon:nitrogen (C:N) ratio maintained at optimum level (3:1) with monochlorophenols and growth substrates. The organism clumped when grown in the presence of monochlorophenols alone. The degree of clumping decreased with the addition of growth supplements.

Keywords: Biodegradation, Growth supplement, Monochlorophenol, Pseudomonas putida CP1

DOI: http://dx.doi.org/10.3329/bjm.v24i2.1254

 Bangladesh J Microbiol, Volume 24, Number 2, December 2007, pp 115-118