Comparative analysis for the detection of tuberculosis by conventional and flurosence microscopy

Authors

  • Ohiuddin Ahmed National Tuberculosis Reference Laboratory (NTRL), National Instiute of Diseases of the chest and hospital (NIDCH), Mohakhali, Dhaka,
  • Zakaria Ahmed Associate Professor, Department of Microbiology, Faculty of Biological Science Primeasia University, 9 Banani Dhaka-1213,

DOI:

https://doi.org/10.3329/bjms.v12i3.11104

Keywords:

Comparative analysis, TB, Auramine-O and Ziehl-Neelsen staining

Abstract

Total of 300 TB patients sample were collected from NIDCH (Indoor and Outdoor)  for six months period where three different specimens (sputum, pus, BAL) were collected among different age group (male and female). In sputum sample, percentage of identified samples in Auramine-O stain  was 41% whereas in Z-N stain 32%;  in pus sample, Auramine stain is 22% and Z-N stain 15%; In BAL sample,  Auramine stain is 12% and Z-N stain is 10%. Therefore, out of 300 samples, 25% of positive samples were identified by Auramine-O staining whereas 19% of positive samples were identified by Z-N stain. In all the cases Auramine-O is found better for identification of TB than Z-N.  In sputum, male positive 10%, female positive 3.66%; in pus sample, male positive 5% and female positive 2.33%; in BAL sample, male positive 3% and female positive 1%. Age group of 26 to 45 years were found high rate of TB in male patients.

Bangladesh Journal of Medical Science Vol. 12 No. 03 July 13 Page 315-319

DOI: http://dx.doi.org/10.3329/bjms.v12i3.11104

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Published

2013-06-23

How to Cite

Ahmed, O., & Ahmed, Z. (2013). Comparative analysis for the detection of tuberculosis by conventional and flurosence microscopy. Bangladesh Journal of Medical Science, 12(3), 315–319. https://doi.org/10.3329/bjms.v12i3.11104

Issue

Section

Case Reports