Use of fluorescein diacetate (FDA) staining to detect viable Mycobacterium tuberculosis

Authors

  • Shamima Islam BRAC Health Program, Regional Tuberculosis Reference Laboratory (RTRL), General Hospital, Chittagong
  • Farjana Rahman Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217
  • Saurab Kisore Munshi Lecturer, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217
  • Jewel Ahmed University Research Co., LLC (URC), Banani, Dhaka-1213
  • S M Mostafa Kamal National Tuberculosis Reference Laboratory (NTRL), NIDCH, Mohakhali, Dhaka-1212
  • Rashed Noor Associate Professor & Chairman, Department of Microbiology, Stamford University

DOI:

https://doi.org/10.3329/bjms.v11i4.12605

Keywords:

Tuberculosis, fluorescein diacetate staining, auramine O staining, Lowenstein-Jensen culture, multi-drug resistance

Abstract

Objective: Drug resistant tuberculosis has long been a common problem prevailing in developing countries including Bangladesh. Present study focused on the rapid identification of live Mycobacterium tuberculosis among treatment failure cases.

Materials and Methods: Sputum samples from a total of 100 category-I and category-II treatment failure cases, assumed as multidrug resistant tuberculosis, were studied through fluorescein diacetate (FDA) staining under light emitting diode (LED) fluorescence microscope. Considering culture method as gold standard, we also compared the results of FDA staining with that of auramine O staining.

Results: A total of 85% acid-fast bacilli were detected by FDA staining, 82% by auramine O staining and a total of 85% isolates were detected in Lowenstein-Jensen (LJ) culture. The sensitivity of FDA staining (96.47%) was estimated to be slightly higher than that of auramine O staining (91.76%). Moreover,76.47% cases were detected as multidrug resistant tuberculosis (MDR-TB).

Conclusion: Taken together, FDA staining method has been proposed to be appropriate for the rapid diagnosis of drug resistant tuberculosis.

DOI: http://dx.doi.org/10.3329/bjms.v11i4.12605

Bangladesh Journal of Medical Science Vol. 11 No. 04 Oct12

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Published

2012-11-13

How to Cite

Islam, S., Rahman, F., Munshi, S. K., Ahmed, J., Kamal, S. M. M., & Noor, R. (2012). Use of fluorescein diacetate (FDA) staining to detect viable Mycobacterium tuberculosis. Bangladesh Journal of Medical Science, 11(4), 322–330. https://doi.org/10.3329/bjms.v11i4.12605

Issue

Vol. 11 No. 4 (2012)

Section

Original Articles

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