Impact of promoter CD14 C>T 159 gene single nucleotide polymorphism and outcome of sepsis

Authors

  • Sumaira Binti Bashir Department of Anaesthesia and Critical Care, SKIMS, Srinagar (J&K)
  • Abdul Qayoom Dar Department of Anaesthesia and Critical Care, SKIMS, Srinagar (J&K)
  • Roohi Rasool Department of Immunology and Molecular Medicine, SKIMS, Srinagar (J&K)
  • Rafia Shamim Department of Anaesthesia and Critical Care, SKIMS, Srinagar (J&K)
  • Arshad Pandit Advanced Centre for Human Genetics, SKIMS, Srinagar (J&K)

DOI:

https://doi.org/10.3329/bjms.v15i4.30715

Keywords:

Sepsis, CD 14, Single Nucleotide Polymorphism (SNP), Outcome

Abstract

Introduction: A genetic polymorphism has been identified inside the CD14 Promoter sequence. It consists of a C to T transition at base pair -159 from the major transcription site. Subjects carrying the T allele have been shown to have significantly higher soluble CD14 levels than do carriers of the C allele. Consequently, genetic variations in CD14 particularly polymorphism located on the promoter region are thought to have functional effects and increased susceptibility to sepsis.

Methods: Our study was a case control study in which a total of 85 samples were included out of which 50 were sepsis free controls and 35 cases of sepsis. Both the cases and controls were selected from Surgical Intensive Care Unit of Sher-i-Kashmir Institute of Medical Sciences (SKIMS), Srinagar. Age more than 80 years, cardiac failure, liver insufficiency and cancer patients were excluded from the study. 5ml of blood from peripheral vein was obtained from each subject in EDTA containing vials and DNA extraction was done by salting out method.

Results: The TT genotype frequency was significantly higher in sepsis than in control (P=0.025) and appeared to be genetic risk factor for increased susceptibility to sepsis. The frequency of mutant T allele observed in cases was 36(51.4%) and 35(35%) in controls. This observation showed a highly statistically significant of rare allele T between cases and controls (P=0.033). Patients with increased Total Leucocytes Count (TLC) were more significantly associated with combined (CT+TT) against CC against those patients with normal TLC (P<0.01). The cases had a higher frequency of the rare allele (CT+TT = 80%) than the controls (64%) and this difference showed statistically insignificant association with CT + TT combination against CC (P=0.11).

Conclusion: The present study suggested that CD14-159C>T may be a risk factor for the development of sepsis in Kashmiri Population.

Bangladesh Journal of Medical Science Vol.15(4) 2016 p.538-545

Downloads

Download data is not yet available.
Abstract
25
PDF
25

Author Biography

Sumaira Binti Bashir, Department of Anaesthesia and Critical Care, SKIMS, Srinagar (J&K)



Downloads

Published

2016-12-18

How to Cite

Bashir, S. B., Dar, A. Q., Rasool, R., Shamim, R., & Pandit, A. (2016). Impact of promoter CD14 C>T 159 gene single nucleotide polymorphism and outcome of sepsis. Bangladesh Journal of Medical Science, 15(4), 538–545. https://doi.org/10.3329/bjms.v15i4.30715

Issue

Vol. 15 No. 4 (2016)

Section

Original Articles

License

Authors who publish in the Bangladesh Journal of Medical Science agree to the following terms that:

  1. Authors retain copyright and grant Bangladesh Journal of Medical Science the right of first publication of the work.

  2. Creative Commons Licence
    Articles in Bangladesh Journal of Medical Science are licensed under a Creative Commons Attribution 4.0 International License CC BY-4.0.This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
  3. Authors are able to enter into separate, additional contractual arrangements for the distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  4. Authors are permitted to post their work online (e.g., in institutional repositories or on their website) as it can lead to productive exchanges, as well as greater citation of published work.